Expt 13 Study of stereotype and anti-catatonic activity of drugs on rats/mice.

For educational purpose only

1. AIM: Study of stereotype and anti-catatonic activity of drugs on rats/mice . Presented by: Prof. Mirza Anwar Baig Dept of Pharmcaology AIKTC, School of Pharmacy, New Panvel

2. THEORY: Stereotyped movements : Aimless and repetitive movements. Catatonia: L ack of movement and communication, agitation, confusion, and restlessness Mostly associated with antipsychotic drugs like phenothiazine and reserpine . May be due to deficiency of dopamine by blocking of dopaminergic receptors. Parkinsonism  is any condition that causes a combination of the movement abnormalities — such as tremor, slow movement, impaired speech or muscle stiffness — especially resulting from the loss of dopamine-containing nerve cells (neurons).

3. THEORY: Perphenazine is used to induce catatonia and parkinsonism. Anti parkinoism drugs include levodopa , scoplolamine and atropine etc. The aim of this experiment is to assess the activity of anti parkinsonian activity of drugs by drug induced catatonia.

4. Requirement: Animals: Rats 150-200 gm Equipment: Wooden blocks of 3 and 9 cm height Drugs: Perphenazine (5mgkg.I.P) Scopolamine (1mg/kg p.o )

5. Procedure: Weigh the animals and mark them serially. Divide the animals into two groups (control and treatment group) equally. Control group will receive only perphenazine and other group will receive scopolamine and perphenazine both. Scoplamine will be administered 30 mins prior to perphenazine .

6. Procedure Contd … 5. Observe and score the different stages of catatonia. Score 0: when the animal will be kept on the table it moves normally. Score 1: animal moves when it will be puhsed or touched. Score 2: Animal fails to correct its posture when front paws will be set on 3 cm wooden block Score 3: animal fails to remove its front paws when placed on 9 cm wooden block Observe and score the activity of animals of both groups after 15 min 30,45,60,90,120 min https://www.youtube.com/watch?v=Y-HfB5UO7bc https://www.youtube.com/watch?v=JwvzaeMXrWM

7. Observation SR. NO Weight of animal (g) Dose Dose to be administered Mean Catatonia score after min of PERP Treatment 15 (min) 30 (min) 45 (min) 60 (min) 90 (min) 120 (min) 1 180 Perphenazine (5 mg/kg) 0.9 0.5 1 3 2 0.5 2 185 0.92 1 3 2 1 3 205 1.02 0.5 0.5 1 3 2 2 4 210 1.05 0.5 1 1 3 2 1 5 200 1 1 2 3 1 Mean   0.2 0.4 1 2.8 2.1 1.1 G1: CONTROL ( Perphenazine )

8. G2:Treatment SR. NO Weight of animal (g) Dose Dose to be administered Mean Catatonia score after min of PERP Treatment SCOP (Mg) PERP (Mg) 15 (min) 30 (min) 45 (min) 60 (min) 90 (min) 120 (min) 1 185 Scopolamine (2 mg/kg) + Perphenazine (5 mg/kg)     1 1 2 190     1 1 3 200     1 2 1 4 205     2 1 5 195     1 1 1 1 Mean     0.6 1.4 0.6 0.2 Group Mean Catatonia score after min of PERP Treatment 15 (min) 30 (min) 45 (min) 60 (min) 90 (min) 120 (min) Control (Perphenazine) 0.2 0.4 1 2.8 2.1 1.1 Test (Scopolamine + Perphenazine) 0.6 1.4 0.6 0.2

9. Results (Bar Graph)

10. Result: Scoplamine decreases the mean catatonic score, It may have anti- catatonic activity .

11. Application in research:

12. References: Ghosh MN. Fundamentals of Experimental Pharmacology. Hilton & Company, Kolkata. Kulkarni SK. Handbook of experimental pharmacology. VallabhPrakashan

13. Thank You