Factors affecting fluorescence intensity(pharmaceutical analysis)

Pharmaceutical Analysis part-1

1. FACTORS AFFECTING FLUORESCENCE INTENSITY Name:-Md. Irfan Uddin Roll:-1083 Reg no:-1086 Session:-Jan-2018 University of Science and Technology Chittagong

2. Content 2 Introduction Principle of Fluorometry Factors affecting fluorescence intensity Conclusion

3. Introduction 3 FLUORESCENCE FLUOROMETRY Fluorescence  is the emission of light by a substance that has absorbed light or other  electromagnetic radiation . In most cases, the emitted light has a longer  wavelength , and therefore lower energy, than the absorbed radiation . It is measurement of fluorescence intensity at a particular wavelength with the help of a filter fluorimeter or a spectrofluorimeter .

4. 4 Principle of Fluorometry

5. Factors affecting fluorescence intensity 5 Intensity of fluorescence is directly proportional to the rigidity of structure.  So,rigid structures shows more fluorescence Intensity, flexible structures shows less fluorescence Intensity Rigidity of Structure Rigidity of Structure  FI

6. Continued… 6 Intensity of fluorescence is inversely proportional to the increasing of temperature. So,increasing temp. leads to increase collision of molecule decrease FI. decreasing temp. leads to decrease collision of molecule increase FI. Effect of T emperature Increasing temp.   

7. Oxygen decreases the fluorescence intensity by oxidises fluorescent substance to non-fluorescent substance. Continued… 7 Effect of Oxygen Effect of P H P H depends on chemical structure of molecule. Phenol:- Acidic condition undissociated NO FI Alkaline condition dissociated FI

8. Continued… 8 Conjugation system

9. Continued… 9 Nature of Substituent group

10. Continued… Fluoroscence Intensity is directly proportional to concentration of substance.But, this is true in low conc. ( 50 g/ml).But in high conc. ( 50 g/ml) it does not obey linearity. Effect of Concentration

11. Continued… Presence of other solutes in solution which are non fluorescent can also affect fluorescent intensity by the so called inner filter effect. Inner Filter Effect Viscosity

12. Conclusion Fluorometry is a quantitative analysis technique. Several factors affects the intensity of fluorescence among them conc. Plays vital role.According to BEER’S law firstly it shows linear curve that means direct proportional but after certain conc. It shows deviation that times increasing conc. Can’t increase the FI.

13. References Sharma Y R. Elementary organic spectroscopy . 16th ed . s chand &company Ltd. New dellli 2007.  Gurudeep R chatwalSham K . lnstrumental method chemical analysis . 5th ed. Himalaya publishing house. Mumbai 2002 p .2.399-2.41 G.  Ravi Sankar . Text boook Of pharmaceutical analysis .3'd ed. Rx publica tion. 2006.  http ,// images.google.co.in/imghp?oe-üff- 8&hI-en&tab-wi&q-fluorescence  httpt// www.bertholdtech.com/ww/en pub/bioanalytik/biomethods/fluor.cfm