Final Labs Unmarked B&W.pdf histology images

Histology images

2. Compact Bone- ground
-white circular structures are the central canals
(contains neurovascular bundles)
-"onion ring” structures surrounding are the

osteons

4. -White arrows: osteons
-small black: interstitial lamellae (random
arrangement)

-bold black: external circumferential lamellae

6. -white octagon: osteon
B) tiny “hair structures"—> canaliculi
A) concentric lamellae

-black arrow: interstitial lamellae

8. -left: lacuna
-middle: Haversian canal (center canal)

-right: canaliculi

10. A) Haversian canal
B) canaliculi

C) lamellae

12. Compact bone (injected ink)
A) perforating (Volkmann's) canals

B) central canal

14. A) Volkmann's (perforating) canals

B) central canal

16. Decalcified compact bone
-H&E + Bright-field LM

18. Compact bone
A) Haversian canal

B) Volkmann's canal

20. Spongy bone- articular surface
Decalcified
A) hyaline cartilage
B) trabeculum
Notice in the trabeculum we have dark (red)
and light (yellow)
Dark areas- red bone marrow

Light areas- yellow marrow rich w/ adipocytes

22. Spongy bone- vertebrae
A) red bone marrow

B) spicules

24. Decalcified
-H&E Stain + bright-field
A) bone marrow
B) spicules

-arrow on top: mesenchyme

26. Decalcified
A) osteoblast cell
B) osteocyte
C) bone marrow cavity

(Very top part- mesenchyme)

28. A) osteoblast
B) blood vessel (need lots of supply because
we're forming new tissue)
C) marrow cavity
(Side note: look at the osteoblast arrows- the
cells are bigger. Now look at the cells around
the marrow cavity, theyre flattened -> bone

lining cells)

30. A) bone marrow
B) osteoclast
C) osteoblast
D) Howship lacunae

E) osteocyte

32. Hyaline cartilage in the trachea
A) pseudostratified columnar ciliated
epithelium
B) perichondrium
-that big oval shape in the top left is a blood
vessel

-the little “bubbles” are isogenous groups

34. Hyaline cartilage
A) chondrocytes
B) chondroblasts (faces the perichondrium)
-territorial matrix: darker staining, rich w/ GAGs.
Immediately surrounds lacunae
-interterritorial matrix: lighter (eosinophilic

matrix), rich w/ collagen & less GAGs

36. A) chondrocyte in its lacunae
B) territorial matrix
C) interterritorial matrix
D) chondroblasts

38. A) territorial matrix
B) interterritorial matrix

C) lacunae

40. Hyaline cartilage (trachea)
-staining appears less-> due to
-arrow: perichondrium

“empty space” in the middle -> artifact

42. A) chondrogenic cells (chondroblasts)

B) nest (lacunae)

44. Articular cartilage (surfaces of the joints)

-no perichondrium

46. Articular cartilage (Surface of joints)

48. Elastic cartilage

-black arrow: perichondrium

50. Elastic cartilage (higher magnification)
A) chondrogenic (chondroblasts)

B) chondrocytes in lacunae

52. Elastic cartilage

54. Fibrocartilage (intervertebral disc)
A) vertebrae

B) Hyaline cartilage

55. 4,
/
4

56. Fibrocartilage
A) colllagen

B) chondrocytes in lacunae

58. Fibrocartilage

60. Hyaline cartilage (low magnified)

62. Hyaline cartilage (highly magnified)
-Darker staining areas are collagen Il rich
-Lighter staining areas are collagen | rich

(Clearer with color :/ )

64. Hyaline cartilage (low magnified)

-structure at the top is the perichonrium

66. Hyaline cartilage (highly magnified)

-structure at the top is perichondrium

68. Hyaline cartilage

69. ey

70. Longitudinal section (LS) of skeletal muscle
(Striations are visible= LS)
&E + bright-field LM

-nucleus

72. LS of a skeletal muscle
(Striations are visible= LS)
A) perimysium
B) endomysium

C) muscle cell fiber

74. LS of a skeletal muscle
(Striations are visible= LS)
-| band: lighter regions
-A band: darker regions
-Z disc: center of the | band
-black arrow w/ blue star: nucleus of a fibrocyte
(slimmer)

-blue arrow: nucleus of the muscle fiber

76. Cross section of a skeletal muscle
-the gap is usually where the perimysium is
(Striations are not visible= CS)

-why skeletal?

A) nucleus is peripheral
B) fibers are big

C) packed muscle fibers

78. Skeletal muscle (tongue)
H&E
A) longitudinal section

B) cross section

80. Skeletal muscle
A) capillary
B) cross section- the grainy sections are due to
the myofilaments

C) longitudinal section (striations)

82. Longitudinal section of smooth muscle
H&E
(LS of smooth= you can see the full length of
the nucleus)
-why not skeletal? because the nuclei are not

peripherally located & they are spindle-shaped

84. LS of asmooth muscle (higher magnification)
(LS of smooth= you can see the full length of

the nucleus)

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86. Cross section of smooth muscles

(CS of smooth muscle= nuclei are small circles)

88. Cross section of smooth muscle (higher
magnification)
(CS of smooth muscle= nuclei are small circles)
-notice how the nuclei are larger than the

cytoplasm

90. Smooth muscle
A) CS- “small circular” nuclei

B) LS- spindle shaped long nuclei

92. Smooth muscle (higher magnification)
A) CS- “small circular” nuclei

B) LS- long, spindle-shaped nuclei

94. Wall of blood vessel= smooth muscle
A) vessel (location of smooth muscle)
B) artery & vein-
-artery is on the bottom- thicker walls & smaller
lumen
-vein is on the top right- thinner walls & bigger

lumen

96. Arrector pili muscle- smooth muscle
-gland is sebaceous
-LS

98. Arrector pili muscle (higher magnification) -
smooth muscle
-gland is sebaceous
-LS

100. A) smooth muscle (cut is more LS)
B) skeletal muscle (LS- peripheral nuclei &

fibers are big)

102. Cardiac muscle
H&E
-LS (you can see the fiber length)
-cells branch (look at black arrows) & attach to
other cell fibers

-nuclei are centrally located

104. Cardiac muscle
-CS
-notice how the nuclei are centrally located,

that's what differentiates it from skeletal muscle

106. A) nucleus
B) LS of cardiac muscle
C) intercalated disc (dark eosinophilic

structures- rich w/ gap junctions)

108. Cardiac muscle

-arrow is pointing at the intercalated discs

110. Neuron (®)- multipolar
Toluidine blue stain
A) axon (only one)

B) dendrites (two processes that aren't the axon)
-most of nuclei around neurons are glial cells
-other dark structures are also neurons, but the
section didn't pass through the nucleus

-patches inside the neuron- Nissl bodies

112. Multipolar neuron
Toluidine blue stain
A) neuropil = neuron/glia processes, at their
distal ends they appear thin (kind of resemble
collagen fibers)
B) dendrite
C) glial cells

114. Cross section of a nerve (v. low magnification)
H&E
A) endoneurium
B) perineurium
C) epineurium (dense irregular CT- collagen
fibers & fibrocytes), extends deeper between
fascicles

-circular structures are fascicles

116. Fascicles (more magnified)

-inside the fascicles are myelinated axons w/
Schwann cells & CT wrap (endoneurium-
mainly reticular fibers)

A) endoneurium
B) perineurium: made of layers of fibrocytes w/
junctional complexes in between (well-
adhered)

118. Inside the fascicles
A) Schwann cells- bigger nuclei are for
Schwann cells whereas the smaller nuclei are
for fibrocytes
B) axons- eosinophilic round structure
C) myelin- whitish areas are where myelin
sheath used to be (dissolved in histological

preparations)

120. Cross section of a fascicle
-on the peripheral: perineurium (2-6 layers of
fibrocytes)
-three arrows are pointing at the myelinated axon
-darker region is the axon
-lighter region was occupied by myelin
-nuclei are for Schwann cells

-elongated thin nuclei are for fibrocytes

122. More magnified CS of fascicles
-black arrow: myelin
-gray arrow: axon
-white arrow: Schwann cell nuclei
-blue dotted: surrounding each myelinated
axon are reticular fibers where the endoneurium

is

124. LS of nerve fibers
A) Schwann cells
B) myelin (foamy/washed out)
C) axon (eosinophilic streak in between the
myelin)

D) node of ranvier

126. LS in nerve fibers
Osmium tetroxide (Dr. Ghada said in the in-person lec. that
this WILL come on the test)
-this stain dissolves in fats & forms a black-reduction
compound (appears black)
A) node of Ranvier
B) myelin sheath
-the axon is the white structure between the black streaks

(myelin)

128. Axon cylinder
Osmium tetroxide
A) axons
B) myelin

C) nodes of Ranvier

130. Cross section of nerve fibers
Osmium tetroxide
-small & big myelinated axons
-some have lots of myelin, some have small
amount
-blackish structures are myelin
-around myelin is where endomysium is

supposed to be

132. Cross section of nerve fibers but more
magnified
Osmium tetroxide
-axons in white

-myelin in black

134. Spinal cord

Crystal stain

136. Spinal cord
Crystal stain
-green: white matter
-yellow: gray matter
-blue: motor neurons in the ventral horns

-red: interneurons in the dorsal horns

138. Anterior horns of the spinal cord
Crystal staining
-neurons (0) are motor neurons (multipolar)
-nuclei around the motor neurons are glial cells

-outside (on the far right) is the white matter

140. Central canal of the spinal cord
Crystal staining
-in the gray matter
-darker structures surrounding the CC are the

ependymal cells: neuroglia that secrete the CSF

142. Dorsal root ganglia
-red: ganglia

-blue: spinal cord

144. Dorsal root ganglia (more magnified)
Pseudounipolar neurons
-bigger structures are neurons w/ their

supporting glia (satellite cells)

146. Sensory ganglion
-big structures are the neurons (0)

-surrounding cells are satellite cells

148. Sensory ganglion
-left arrow: cell bodies

-right arrow: satellite cells

150. Dorsal root ganglia (low magnification)
-neurons are the exceptionally large structures

-nerve fibers are the lighter stained regions

152. Dorsal root ganglia (higher magnification)
Pseudounipolar
-large structures are neurons
-nuclei around them belong to satellite cells

-on the right, those are axons

154. Spinal cord
-yellow: spinal cord in the vertebral canal
-red: dorsal root ganglia which contain the cell
body of sensory neurons (on both sides)

-green: autonomic ganglia (sympathetic)

156. Dorsal root
-structure on the further right: spinal cord
-large structures: neurons
-next to the neurons we have nerve fibers

-top left structure: skeletal muscle

158. Autonomic ganglia (green circle a few slides
ago)

-has CT covering
-postganglionic sympathetic neurons
(multipolar)

-preganglionic neurons are in the CNS (spinal

cord)+ pseudounipolar