Formulation Of Poly Herbal Ointment all final (2).pptx

formulation for short term project

1. Formulation Of Poly Herbal Ointment And Evaluation Of Its Antimicrobial Activity

2. 2 CATALOG

3. POLY HERBAL OINTMENT Poly Herbal Ointment Is A Type Of Topical Medication Made From A Combination Of Several Herbs Or Plant Extracts. It Is Used To Treat A Variety Of Skin Conditions Such As Eczema, Psoriasis, Rashes, And Fungal Infections. The Combination Of Herbs Used In The Ointment Is Chosen Based On Their Therapeutic Properties, Which May Include Anti-inflammatory, Anti-bacterial, Anti-fungal, And Anti-viral Effects. Poly Herbal Ointments Can Be Formulated As Creams, Gels, Or Balms And Are Usually Applied Directly To The Affected Area Of The Skin. They Are Often Preferred Over Synthetic Medications Due To Their Natural Origin, Minimal Side Effects, And Potential For Synergistic Effects Among The Herbs Used.

4. Piper Betle Betel Plants Are Cultivated For Their Leaves Which Are the Most Commonly Used As Flavoring In Chewing. Commonly Found In Malaysia,Vietnam ,  Cambodia, Thailand, And Myanmar. Chemical Constituents Present Chavibetol ,Safrole Eugenol, Isoeugenol USES 1. Helps In Wound Healing 2. Improves Oral Health 3. Anti Bacterial 4. Anti Inflammatory Kingdom: Plantae Division: Magnoliophyta Class: Magnoliopsida Order: Piperales Family: Piperaceae Genus: Piper Species: P. Betle 4

5. Tagetes Erecta There Are Three Main Types Of Marigolds: French, African, And Signet.  African Marigolds Can Reach 3-4 Feet. French Marigolds ( Tagetes Patula ) Are Usually Between 6-12 Inches Tall. Signet Marigolds ( Tagetes tenuifolia ) Are The Smallest, Usually 6 Inches Or Less. Kingdom : Plantae Division : Magnoliophyta Class :Magnoliopsida Order : Asrerales Family : Calenduleae Genus :Calendula Uses Beneficial In Treating Ulcers, Piles, Kidney Problems, And Muscle Pain. Heals Skin Wounds, Burns, And Rashes. Insect repellent

6. Carica Papaya The Papaya Is A Small, Simply Branched Tree, Usually With A Single Stem Growing From 5 To 10 M. Papaya Skin, Pulp, And Seeds Contain A Variety Of Phytochemicals, Including Carotenoids  And   Polyphenols. Kingdom: Plantae Phylum: Tracheophyta Class: Magnoliopsida Order: Brassicales Family: Caricaceae Species: Carica Papaya Uses It Is Used To Reducing Inflammation, Improving Blood Sugar Control, Supporting Skin And Hair Health, And Preventing Cancer.

7. Ixora coccinea  (Also Known As Jungle Geranium). Commonly Found In Southern India, Bangladesh And Sri Lanka. The Flowers, Leaves, Roots, And The Stem Are Used To Treat Various Ailments In The Indian Traditional System Of Medicine. Chemical Constituents Present Ursolic Acid, Oleanolic Acid, Sitosterol . Kingdom: Plantae Phylum: Magnoliophyta Class: Magnoliopsida Subclass: Asteridae Order: Gentianales Family: Rubiaceae Genus: Ixora Subject: Ixora coccinea L 7 Uses Antioxidative , Antibacterial, Gastroprotective , Hepatoprotective, Antidiarrhoeal , Antinociceptive , Antimutagenic , Antineoplastic, And Chemopreventive Effects. Used In Ayurveda

8. The Most Widely Grown Species Of Annona And A Native Of Tropical Climate In The Americas West Indies, And In India. Chemical Constituents presents tannins, alkaloids, phenols, glycosides, flavonoids and steroids Pharmacological Properties And Biological Activities, Such As  Antioxidant, Antimicrobial, Antidiabetic, Antiviral, Anticancer, And Hepatoprotective Activities. Kingdom: Plantae phylum: Tracheophyta Class : magnoliopsida Order:  Magnoliales Family:  Annonaceae Genes : Annona Species: Annona reticulate L 8 Annona Reticulata Uses The Plant Is Traditionally Used For The Treatment Of Epilepsy, Dysentery, Cardiac Problems, Worm Infestation, Constipation, Hemorrhage, Antibacterial Infection, Dysuria, Fever, Ulcer

9. 9 Collection Extraction Formulation of ointment Antibacterial activity Materials And Methods

10. collection First We Collected Some Herbal Leaves. Then We Shade Dried For 12 To 14 Days Before Being Ground Into Course Powdered It Was Kept In A Well Sealed Container Away From Climatic Changes Until Use. 10

11. Extraction 11

12. Formulation of Ointment S. No Name of the ingredient Quantity to be taken 1 Cetostearyl alcohol 15 gms 2 PEG 20ml 12 S. No Name of the ingredient Quantity to be taken 1 Annona reticulata 0.8 gms 2 Carica Papaya 1 gms 3 Ixora coccinea 1 gms 4 Piperbetle 0.76 gms 5 Tagetes erecta 0.95 gms Table 1: Formulation of ointment base Table 2: Formulation of Herbal ointment The Fusion Method Is Used To Prepare The Ointment

13. Influence 13 EVALUATION OF ANTIBACTERIAL ACTIVITY Anti-microbial Activity Is The Practice Of Eradicating Or Preventing The Development Of Microorganisms. Antimicrobial Agents Either Kill Bacteria (Bactericidal) Or Prevent The Development Of Bacteria (Bacteriostatic). Pour Plate Method Preparation Of The Media Of The Media Preparation Of The Sample . Take nutrient agar in a conical flask add distilled water to it. . Close the conical flask with cotton ball. . Then autoclave it for 15min at 121˚c. Take 1 gm of prepared polyherbal ointment in a 9ml of solvent and dissolve it (serial dilution) . Take 1gm of marketed product ( cetrimide )& in a dissolve in a 9ml of methanol. . Preparation of blank

14. INOCULATION Using A sterile micropipette or calibrated pipette, place 1 ml of the diluted sample in the middle of the Petri plate. Flame the bottle's mouth by removing the lid. 15 ml of the sterilized, molten medium at the proper temperature should be poured over the sample.  Then add the pseudomonas aeruginosa After securing the plate's cover, gently spin the plate to properly combine the sample with the medium. Often, A "S" or "8" form is swirled into the plate. . Place the plate in an inverted position and incubate it under the proper incubation conditions (typically 24 hours at 37°C). 14

15. 15 RESULT INTERPRETATION OF POUR PLATE METHOD Colonies that have formed after the incubation are seen. There will be one viable microbial cell or one colony-forming unit per colony. If every colony belongs to the same kind, we can infer that there was only one type of microbial genus present in the sample. It's possible that various genera or species produce colonies of a similar kind. Count the colonies and calculate CFU/mL by using the formula This will provide the total number of living microorganisms in the provided sample.   Dilutions Ointment Standard (Neosporin) Blank 10 1 3.5×10 3 2.9×10 3 5.5×10 3 10 2 3.8×10 4 2.3×10 4 6×10 4 10 3 3.4×10 5 1.5×10 5 4.9×10 5 10 4 2.7×10 6 1.3×10 6 3.5×10 6 10 5 1.4×10 7 1×10 7 2.6×10 7 10 6 1×10 8 0.5×10 8 1.8×10 8

16. 16 Disk Diffusion Method On The Culture Medium, The Bacterial Culture Was Added, And A Well Was Bored Through The Centre Of The Agar. After That, Various Samples And 0.05ml Standard Solutions Were Added To These Wells, And The Plates Were Then Incubated At 37°C Overnight For Observation. Inhibition Was Seen And Contrasted With The Control. By Examining The Zone Of Inhibition Surrounding Each Well, The Test Organism's Sensitivity To The Plant Extract Under Test Was Identified. Different Samples Zone diameter in mm(pseudomonas aeruginosa) Ointment 13.2±2.3 Standard 11.67±0.23 Blank 21.50±0.71

17. Preparation Of Leaf Extract Each Plant Had Powdered Its Shade Dried Leaves. 100 Ml Of Solvent Was Added To 10 Gms Of Leaf Powder From Each Plant, Which Was Then Left To Sit For Three Days. With The Use Of Whatman No. 1 Filter Paper, The Extract Was Purified, And The Filtrate Was Then Collected. Supernatants Were Obtained After A Second Extraction Of The Residue Twice (With A Three-day Delay Between Each Extraction). At Room Temperature (28 ± 1 C), The Supernatants Were Collected And Slowly Evaporated Until Only 100 Ml Remained. Five Plants' Leaves Were Extracted Using Water Solvents, And The Resulting Extracts Were Then Put In Airtight Bottles For Future Examination. Fourier Transform Infrared Spectrophotometer (FTIR) The Fourier Transform Infrared Spectrophotometer (FTIR) Is Perhaps The Most Effective Instrument For Determining The Kinds Of Chemical Bonds (Functional Groups) Present In Compounds. The Annotated Spectrum Shows That The Wavelength Of Light That Is Absorbed Is Indicative Of The Chemical Bond. A Molecule's Chemical Bonds Can Be Identified By Reading The Infrared Absorption Spectra. Ftir Analysis Was Conducted Using Dried Powdered Water Solvent Extract Of Plant Components. To Create Transparent Sample Discs, 100 Mg Of Kbr Pellet And 10 Mg Of The Dried Extract Powder Were Combined. Each Plant Specimen's Ground-up Sample Was Placed Into An FTIR Spectroscope With A Scan Range Of 400 To 4000 Cm-1 And A Resolution Of Cm-1 . Phytochemical Analysis

18. 18 Extracts Peak Values Functional Groups Piper Betle 3390 2931 1600 1384 1191 1122   Oh C-h C=o Ch2 C-o C-o Tagetes Erecta 3308 2942 1772 1599 1420 1080   C-o C-h C=o C=c Ch2 C-o Carica Papaya 3412 1614 1390 1117 O-h, N-h C=o Ch2 C-o Ixora coccinea 3374 2934 1608 1384 1121 O-h C-h C=o Ch2 C-o Annona Reticulata 3306 2927 1604 1419 1076 O-h C-h C=o Ch2 C-o

19. 19 FTIR spectrum of water extract of Ixora coccinea FTIR spectrum of water extract of Tagetes Erecta FTIR spectrum of water extract of Annona Reticulata FTIR spectrum of water extract of Carica Papaya FTIR spectrum of water extract of Piper Betle

20. 20 References 1.https://www.researchgate.net/publication/224898519_Anti_Microbial_activity_of_the_various_leaf_extracts_of_Vitex_negundo_Linn 2.https://www.slideshare.net/SriramNagarajan19/antibacterial-activity-of-extracts-of-tachyspermum-ammi-fruits 3.https://www.researchgate.net/publication/284392560_Preparation_and_Evaluation_of_Antimicrobial_and_Antioxidant_Activity_of_Polyherbal_Ointment   4.https://www.researchgate.net/publication/363049573_Formulation_and_Evaluation_of_Polyherbal_Cream 5.file:///C:/Users/STUDENT/Downloads/3-Vol.-4-Issue-12-December-2013-IJPSR-1108-Paper-3.pdf 6.https://globalresearchonline.net/journal contents/v38-2/34.pdf 7.file:///C:/Users/STUDENT/Downloads/10.11648.j.sjph.20170506.11.pdf 8.https://www.pharmatutor.org/articles/a-review-on-pharmacological-aspects-of-tagetes-erecta-linn 9.https://www.researchgate.net/publication/307904829_A_Brief_Study_on_Carica_Papaya-_A_Review 10.file:///C:/Users/STUDENT/Downloads/16-Vol.-10-Issue-4-Apr-2019-IJPSR-RA-10610.pdf 11.file:///C:/Users/STUDENT/Downloads/Manjeshwar.pdf 12.https://www.ijrap.net/admin/php/uploads/1380_pdf.pdf 13. file:///C:/Users/STUDENT/Downloads/5-Vol.-5-Issue-1-Jan-2014-IJPSR-1121-Paper-5.pdf 14.https://www.researchgate.net/publication/288628769_An_overview_on_medicinally_important_plant_-_Annona_reticulata_linn 15.https://www.researchgate.net/publication/278050304_Annona_reticulata_Linn_Bullock's_heart_Plant_profile_phytochemistry_and_pharmacological_properties 16.https://www.ijcmas.com/vol1/R.Ashokkumar%20and%20M.%20Ramaswamy. pdf

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