FRESH VS FROZEN SPERM your advice? Final

Fresh vs frozen sperm for IVF, ICSI and IUI by Shreya Munshi

1. FRESH VS FROZEN SPERM YOUR ADVICE?

2. DISCLOSURE One of the most crucial developments in reproductive medicine is the introduction of assisted reproductive technology or ART. People can increase their chances of getting pregnant with procedures like intrauterine insemination (IUI) and in vitro fertilization (IVF). Additionally, patients have the option of using fresh or frozen sperm in these procedures.

3. VS

4. INTRODUCTION Sperm samples are an essential part of the  IVF  process. For men with male-factor infertility, the suitable sperm sample can increase the chances of pregnancy. A frozen sperm sample is collected and cryogenically frozen for future use. Frozen sperm can last for several years without losing viability. The frozen sperm is thawed before the fertilization process. A fresh sperm sample is taken from the intended husband and used immediately within 1-2 hours post semen collection. Fresh samples often coincide with the egg extraction process.

5. The ivf process step by step

6. Iui process step by step

7. FRESH SPERMS A sample of male sperm that is taken and used almost right away is known as fresh sperm. The sample is washed and processed in a unique solution to get rid of any impurities like debris or cells that may have negative effect. The sperm is then prepared for IUI (intrauterine insemination) or to create an embryo through IVF.

8. The motility, concentration of the sperm (the number of sperm per unit volume of semen) and most importantly, morphology are thoroughly checked. A concentrated amount of healthy sperm is obtained by separating the sperm from the seminal fluid, and the washed sperm is then used for IUI or IVF. Additionally, some studies show that fresh sperm-derived embryos may have a longer shelf life than frozen sperm. 

9. FROZEN SPERMS Frozen Sperm is a type of sperm that has been cleaned and prepared for freezing before being kept in liquid nitrogen cryotanks for an extended period of time. When the frozen sample is ready to be used, it will be thawed until it reaches room temperature.

10. After thawing, the sample will be examined once more to ensure that there are still healthy and viable sperm present. Sperm that has been frozen can be kept for a long time without losing viability. After thawing it is washed to remove the cryoprotectants. Ideally the rate of thaw survival of sperm is over 50%

11. benefits OF USING FRESH and frozen SPERM SAMPLE

12. PROS of using fresh samples Using fresh sperm samples during the IVF process brings peace of mind to couples. The clinic and patient will know the quality of the sperm. Some studies also reveal that embryos made with fresh sperm may last longer than frozen sperm. Fresh semen has less wastage than frozen. I n frozen sample survival rate of sperm after thawing is less, hence more motile sperms are recovered from fresh sample.

13. PROS of using frozen samples On the other hand, frozen sperm brings convenience. The IVF process becomes more accessible as men can preserve fertility for issues like cancer or patients that have collection issue or erectile dysfunction. F rozen samples are kept for future use. It can be kept as long as you want without degrading its quality. Additionally, frozen sperm is excellent for procedures such as intracytoplasmic sperm injection (ICSI).

14. DRAWBACKS OF USING FRESH and frozen SPERM SAMPLE

15. Cons OF USING FRESH SAMPLE Prepared sample has to be used within 1-2 hours post collection as the capability of sperm to fertilize oocyte decreases as time extends beyond recommended range.

16. Cons OF USING FROZEN SAMPLE Frozen semen has more wastage of sperm than fresh. I n frozen sample survival rate of sperm after thawing is less, hence less motile sperms are recovered from frozen-thawed sample. Cost of freezing is high and if you are renewing your freezing sample, the cost increase with every proceeding year. Conception rate maybe lowered.

17. DOES IT MAKE A DIFFERENCE? Frozen sperm is as effective as fresh sperm for both IUI and IVF. Studies show there is no difference in implantation rates and pregnancy rates. Both samples can help with pregnancy, and more importantly, frozen sperm would not interfere with pregnancy rates.

18. That means IVF patients will have more control over IVF cycles. Thanks to frozen samples, single women and same-sex couples can start families at a more convenient time.

19. For IVF/ICSI

20. Conclusions In this idealized model that controls for oocyte quality to the greatest degree possible by using paired recipients from the same donor from the same stimulation cycle, we found that cycles using frozen sperm resulted in lower live birth rates than those using fresh sperm. These findings suggest that fresh sperm should be preferentially used in non-male factor infertility IVF-ICSI cycles.

21. CONCLUSION This study provides strong evidence to support the notion that motile, cryopreserved and thawed epididymal spermatozoa are equal to freshly retrieved spermatozoa for ICSI in couples with obstructive azoospermia. It is hoped that these findings raise clinical awareness of the value and priority of sperm cryopreservation in all epididymal sperm aspiration procedures.

22. Which Type of Sperm is Best for ART Procedures? The patient's chosen plan and whether they use sperm from a partner, a directed donor, or an unidentified donor will largely determine the sperm used in fertility treatment. Any type of sperm is acceptable, according to the majority of fertility doctors, as long as the donor's or the partner's sperm meets certain standards. More significant than the type of sperm is the health of the patients and the sperm.

23. IUI and IVF are both negatively impacted by the sperm of older men who have mobility and shape issues. The success of IVF cycles can be impacted by sperm quality. It's also crucial to consider a woman's overall health, including her ovarian reserve and uterine lining. It is important to take age and general fertility into account. The sperm's freshness or freezing will be the main factor in these decisions. The aforementioned elements are far more crucial to the likelihood of conception and pregnancy than the type of sperm used in the procedure.

24. conclusion Intra uterine insemination and In vitro fertilization is an excellent way for infertile couples to start a family finally. There is an ongoing concern with the sperm sample, but both are effective. The success rate comes down to other factors like the patient’s health and the expertise of the fertility clinic. Frozen or fresh sperm samples are just a matter of convenience .

25. Take home message According to the majority of studies, using frozen sperm in IVF cycle does not statistically significantly lower the success rate. Age, ovarian reserve, and other genetic disorders are additional variables that can have a significant impact on the success rate. In a study that looked at IUI cycles for people with ovaries who were diagnosed with unexplained infertility, researchers found that fresh sperm led to significantly higher live birth and pregnancy rates when compared to frozen sperm for IUI using ovulation-inducing medications.

26. However, the majority of clinics concur that IUI can be successful with either fresh or frozen sperm. The type of sperm used has no bearing on the factors that are likely to be more crucial for IUI. Age, male infertility, sperm-related factors like sperm health, anatomical factors, and other genetic conditions can all have a significant impact on IUI infertility rates . Frozen sperm is as effective as fresh sperm for IUI and ICSI. Studies show there is no difference in implantation rates and pregnancy rates.