Gel electrophoresis

Gel electrophoresis

1. GEL ELECTROPHORESIS Presented by sweety gupta (M pharm, Pharmacology) BLDEa s ssm college of pharmacy and research centre, Vijayapur Date of Presentation- 25-05-2021 Time of Presentation- 11am-11-30am

2. Contents- Principle of Gel electrophoresis. Instrumentation and Reagents. Working of Gel electrophoresis. Factors affecting separation in Gel electrophoresis. Applications of Gel electrophoresis.

3. Principle- Method of separating DNA, RNA and Protein on the basis of size. It involves electrical field.

4. The molecules travel through the pores in the gel at a speed that is inversely related to their lengths. Means that a small DNA molecule will travel a greater distance through the gel than will a longer DNA molecule.

5. DNA/RNA are negatively charged molecules, pulled towards positively charged end of the gel.

6. 1)

7. Working conditions- Gel electrophoresis of DNA.

8. References- https://www.slideshare.net/Chethanchunkey/gel-electrophoresis-75424834 https://www.khanacademy.org/science/ap-biology/gene-expression-and-regulation/biotechnology/a/gel-electrophoresis#:~:text=Gel%20electrophoresis%20is%20a%20technique,move%20towards%20the%20positive%20electrode.

9. References- https://www.slideshare.net/Chethanchunkey/gel-electrophoresis-75424834