GENE-TRANSFER-METHODS.pptx.BSFLECTURENOTE

About gene transfer and its methods

1. GENE TRANSFER METHODS

2. Direct DNA transfer methods  rely on the delivery of naked DNA into the plant cells. The direct gene transfer can be broadly divided into two categories. A . Physical gene transfer methods E lectroporation   Microinjection P article bombardment .

3. ELECTROPORATION GENE TRANSFER Electroporation can be used to deliver genetic material into cells that are difficult to transfect. Electroporation provides consistent and reproducible results. Electroporation does not require the use of vectors for gene transfer. It is a safe and non-toxic method.

4. Liposome-Mediated Transformation T hese liposomes bind and condense DNA spontaneously to form complexes with high affinity to cell membranes. Endocytosis of the complexes followed by disruption of the endosomal membrane appears to be the major mechanism of gene delivery.