Genetic polymorphism of IL-17 and miRNA-146 in association.pptx

immunology

1. Genetic polymorphisms of IL-17 and miRNA-146 in association with the risk of type 1 diabetes By Nohair Hesham Gomma Mahmoud Faculty of applied medical science Misr university for science and technology

2. Supervisors Dr. Yasser Bastawy Mohamed Associate Professor of Molecular Immunology, genetic engineering and Biotechnology research institute. Prof/Dr. Randa Ghanoum Clinician at National Heart Institution

3. Outlines Identify of DM Identify types of DM Identify of type 1 DM Identify genetic polymorphism in human body Identify cytokines Identify role of IL-17 in type 1 DM Identify miR-146 Identify genetic polymorphisms of IL-17 and miR-146 in type 1 DM Identify aim of our study Identify subjects and methods of our study

4. Diabetes Mellitus Definition : metabolic disorder characterized by hyperglycemia due to an absolute or relative lack of insulin or to a cellular resistance to insulin. Major classification : 1- type 1 2- type 2

5. Type 1 of diabetes mellitus T1D is considered to be a chronic immune-mediated disease with a subclinical prodrome of variable duration. Type 1 diabetes (T1D) is the most common auto-immune disorder in childhood . It is characterized by selective loss of insulin-producing β-cells in the pancreatic islets in genetically susceptible subjects.

6. Type 1 of diabetes mellitus

7. Genetic polymorphism (SNP) Polymorphism: is a genetic term that means “ many shapes ” it is the ability to appear in different form. A single nucleotide polymorphism (SNP): is a DNA sequence variation occurring when a single nucleotide – A, T, C or G- in the genome differs between members of a species (or between paired chromosomes in an individual).

8. Single nucleotide polymorphism (SNP)

9. Cytokines Definition : cytokines are a group of proteins made by the immune system that act as chemical messengers. Different cytokines including: 1- interleukins(IL). 2-interferons (IFN). 3-chemokines. 4- tumor necrosis factor(TNF). Interleukin (IL) There are a large group of cytokines produced mainly by T-cells, although some are also produced by mononuclear phagocytes or by tissue cells. Described as signals for communication between white blood cells . It is well-known that these molecules are produced and used as signaling molecules in many cells of the body ,in addition to immune cells.

10. Role of interleukin-17in T1DM The pathogenic role of IL-17-secreting Th17 cells have been contributed in T1D development through mediate inflammation, by stimulating the production of proinflammatory cytokines, inflammatory chemokines, recruit and activate neutrophils and macrophages. the main function of IL-17 is to magnify inflammatory responses and enhances autoimmune destruction of insulin producing β-cells in the pancreas.

11. miRNA MicroRNAs (miRNAs) are short non-coding RNA molecules usually composed of 18–25 nucleotides. negatively regulate gene expression by partially pairing to the 3′, 5′ untranslated regions of their target mRNAs, leading to translation repression and/or transcript degradation. They have recognized roles in the regulation of various processes, such as cellular differentiation, proliferation, metabolism, aging and apoptosis. miRNA-146 The miR-146 family comprises two genes, miR-146a and miR-146b, which are expressed in response to pro-inflammatory stimuli as negative feedback to control excessive inflammation.

12. Genetic polymorphisms of miRNA-146 and IL-17 in type1DM Genetic polymorphism of miRNA-146: Although the research on miRNAs and T1DM has focused on gene expression studies, polymorphism in miR-146 might be involved in T1DM development since they could alter both the expression and functionality of these miRNA . Genetic polymorphism of IL-17: Single nucleotide polymorphisms (SNP) in IL-17 gene have a significant effect on IL-17 production .

13. The aim of our study Role of genetic polymorphisms of IL-17 and miR-146 in type 1 DM.

14. Subjects and methods One Hundred Patient with T1DM (The inclusion criteria for the group of patients with T1DM were age less than 21 years, normal arterial pressure and no other chronic diseases). One hundred Normal healthy controls have no risk factor for T1DM with age and ethnicity matching to patient subjects.

15. Subjects and methods

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