Comparison Positive Negative After staining Dark blue Pink Peptidoglycan Thick and rigid Thin Teichoic acid Present & consists of water soluble polymers, alcohols and phosphate Absent Outer membrane Peptiglycan Lipoproteins & phospholipids used to protect the cell from mechanical shear Porins Absent Present & Allow the entry of molecules ( i.e ) Disaccharides , B 12 , Amino acid, Nucleotides 4