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1. Basic Microscopy Group 4

2. A microscope is a scientific instrument used to magnify and observe objects that are too small to be seen clearly with the naked eye. It allows scientists, students, and medical professionals to study tiny structures such as cells, microorganisms, and even fine details of materials.

3. Wet Mount Preparation

4. Naked Eye (1x) With the naked eye, the letter “e” is visible in its actual size, but we cannot see its fine details.

5. Scanner (40x) It is used to locate the letter “e” quickly. The scanner has a 40x magnification. In this magnification, we can see the whole letter “e” clearly .

6. Low Power Objective (100x) The low power objective has a 100x magnification. At this level, we can see the letter “e” larger, but not the entire letter fits in the view.

7. High Power Objective (400x) The high power objective has a 400x magnification. In this magnification, only part of the letter “e” is visible, but its details are clearer.

8. In wet mount preparation, why do you need to tilt the cover slip before placing it above the specimen? How is the letter “e” under the microscope different from the “e” On the newspaper? What is its position? How is the letter “e” changing as you move from scanner to HPO? What happens to your focused specimen as you move the stage to the left? To the right? What happens to the viewing field as you increase the magnification? What happens to the resolution as you increase the magnification?

9. Depth of field: Colored thread

10. 4.) What is the Color of uppermost thread? ___________________, middle thread? _______________, lower Thread? _______________. 5.) What happens when you navigated the fine adjustment knob? Can you still focus all three threads? 6.) What happens to the depth of field when magnification was increased?

11. Colored image focusing

12. 7.) Describe the image of the colored specimen as the magnification is increased. VII. Definition of Terms 1.) Interpupillary distance – The distance between the centers of the two eyepieces of a binocular microscope, adjusted to match the user’s eyes for comfortable viewing.

2.) Working distance – The distance between the front lens of the objective and the surface of the specimen when it is in sharp focus.

13. 3.) Magnification – The process of enlarging the appearance of a specimen using lenses, expressed as a multiple of the actual size (e.g., 40x, 100x).

4.) Resolution – The ability of a microscope to distinguish two points as separate and distinct; the clarity or sharpness of the image.

5.) Field of view – The circular area visible through the eyepiece; it decreases as magnification increases.

6.) Depth of view – The thickness of the specimen that remains in focus at one time; higher magnification reduces the depth of view.

14. Thank you!