Histological Techniques: Section 1:Tissue collection and Grossing

Histological Techniques: Section 1: Tissue collection and Grossing

1. Histological Techniques Tissue Collection and Grossing (Section 1) Dr Mathew Joseph MD Anatomy (3 rd year) All India Institute of Medical Sciences, Rishikesh

2. Contents Tissue collection and Grossing: Sources of sampling Instruments Procedure Specimen dissection plan Sampling of tissue outside the laboratory

3. Section 1 Tissue Collection and Grossing

4. Source of Organ/Tissue/Specimen 1. Surgical resection material and biopsy material 2. Postmortem specimens (Preferably recent) 3. Animals: Well perfused experimental / Butchers

5. Workstation Well illuminated room Ventilation and exhaust fan Appropriate sink Waste disposal unit Firm, even and clean surface

6. Instruments Scalpel blades Scissors Probes Stainless steel ruler Forceps Sponges First-aid box with eye wash bottle

7. Procedure Overview Identification of specimen Labelling of specimen Store in normal saline Exposure to air Nervous tissue – Fixative (4 degree C) Avoid water Wash excess of blood and mucous

8. Specimen Dissection Plans SNo Specimen Procedure 1 Small sample Rarely need further dissection 2 Large sample While embedding lay out tissue in longitudinal fashion so that the plane of section cuts along the majority of the tissue: If >4-5 mm divide into two halves along the long axis. 3 Skin Mounted on edge to provide an adequate view of the epidermis, dermis and subcuticular substrates 4 Bowel Sampled with multiple blocks in relation to the adjacent mucosa, wall and serosal aspect tissues. 5 Brain Fixed immediately : Cut into slices once the fixation is done 6 Bone Fixation : Decalcification in EDTA

9. Sampling of tissue outside the laboratory Advanced Breast Biopsy Instrumentation (ABBI) Brush biopsy Computed tomography (CT) Cone biopsy Core biopsy Crosby capsule Curettings

10. Sampling of tissue outside the laboratory Ductal lavage Endoscopic biopsy Endoscopic retrograde cholangiopancreatography (ERCP) Evacuation Excision biopsy Fine needle aspiration Fluroscopy

11. Sampling of tissue outside the laboratory Frozen section Imprint Incision biopsy Liquid based cytology (LBC) Loop electrosurgical excison procedure (LEEP) Magnetic resonance imaging (MRI) Mammography Needle biopsy

12. Sampling of tissue outside the laboratory Positron emission tomography with fluorodeoxy - glucose (PET with FDG) Punch biopsy Sentinel node biopsy Shave biopsy Smears Stereotactic biopsy Transurethral resection

13. Sampling of tissue outside the laboratory Trephine (bone marrow) biopsy Ultrasound Vacuum-assisted biopsies Wire localisation biopsy

14. Advanced Breast Biopsy Instrumentation (ABBI)

15. Thank You