Histology of testes & epididymis

ANATOMY

1. Histology of testis & epididymis ,

2. Histology of testis Testis is covered by ---tunica vaginalis ----tunica albuginea ----tunica vasculosa Tunica albuginea - is a thick white fibrous connective tissue—called capsule of testis Deep to the capsule ,, there is tunica vasculosa – which is a highly vascularised connective tissue. Septa from tunica albuginea divide testis into 200 to 300 lobules Each lobule contains 1- 4 seminiferous tubule Between the seminiferous tubules ,, there is loose connective tissue k/a interstitial tissue & blood vessels

3. lobule seminiferous tubule straight tubule Rete testis Efferent ductules head of epididymis septa

4. Thus--- Glandular portion of testis consist of – 1 seminiferous tubule- a> Spermatogenic cells b> supporting cell of sertoli – also K/A sustentacular cell. 2 Interstitial tissue

5. seminiferous tubule interstitial tissue T.s. of seminiferous tubule

6. seminiferous tubule- Sperm producing tubule Convoluted and long About 30-70 cm long & 200 micro-metre in diameter Surrounded by connective tissue [lamina propria ]----consists of flattened or spindle shaped myoid cells Myoid cells are contractile in nature & help spermatozoa to move through seminiferous tubules.

7. ...... seminiferous tubule- Tubule is lined by stratified cuboidal epithelium Tubule contains-------- i ) ---- spermatogenic cells [ in different stages of spermatogenesis] ii) ---Supporting cells of sertoli

8. spermatogenic cells Spermatogenic cells in 4 successive stage— -- spermatogonia , --primary spermatocyte --secondary spermatocyte --- spermatids . -- primary spermatocyte are the largest cells

9. seminiferous tubule… it contains---- Spermatogenic cells in 4 successive stage— -- spermatogonia , --primary spermatocyte --secondary spermatocyte --- spermatids .

10. supporting cell of sertoli * supporting cell of sertoli – also K/A sustentacular cell . *Tall columnar cell * Its lateral process form a tight junction k/a BLOOD TESTIS BARRIER protects secondary spermatocyte , spermatids & spermatozoa from immune attack

11. BLOOD TESTES BARRIER BLOOD TESTIS BARRIER --secondary spermatocyte BLOOD TESTIS BARRIER

12. Function of sertoli cells Provide mechanical support Provide nutriton to Spermatogenic cells Form blood testis barrier Secrete androgen binding protein Synthesize antimullerian hormone Do phagocytosis of residual body,, degenerating Spermatogenic cells

13. Interstitial tissue occupies space b/w the seminiferous tubules consist of -- Loose connective tissue & -- interstitial cells of Leydig (OVAL CELL). It forms endocrine part of testis Secrete testosterone & a little estrogen

14. low power /T.S. of testis tunica albuginea tunica vasculosa seminiferous tubule spermatogenic cells & Sertoli cell Leydig cells septa

15. low power /T.S. of testis [ nmc slide]

16. epididymis . Highly coiled tub ular structure

17. pathway of sperm from seminiferous tubule seminiferous tubules [highly coiled] in each lobule join to form 20 to30 straight tubules they anastomose to form network of tubules called the RETE TESTIS gives rise to 12to30 EFFERENT DUCTULES which emerge near the upper pole and enter the Epididymis

18. epididymis 1. Highly coiled muscular tubule 2. 6 meter long, made up of smooth muscle 3 . smooth muscle arranged in circular pattern 4. is lined by pseudostratified columnar epithelium with stereocilia .[non-motile cilia] 5. Can store sperms for several months 6. Continues as ductus (vas) deferens smooth muscle pseudostratified columnar epithelium stereocilia sperm in lumen T.S. OF epididymis connective tissue

19. Epididymis -----[ nmc slide]

20. epididymis stereocilia

21. In histo practical class

22. low power /T.S. of testis tunica albuginea tunica vasculosa seminiferous tubule spermatogenic cells & Sertoli cell Leydig cells septa TESTIS

23. TESTIS

24. seminiferous tubule…. b> supporting cell of sertoli – also K/A sustentacular cell . Tall columnar cell Its lateral process form a tight junction k/a BLOOD TESTES BARRIER protects secondary spermatocyte , spermatids & spermatozoa from immune attack

25. epididymis 1. Highly coiled mucular tubule 2. 6 meter long, made up of smooth muscle 3 . smooth muscle arranged in circular pattern 4. is lined by pseudostratified columnar epithelium with stereocilia .[non-motile cilia] 5. Can store sperms for several months 6. Continues as ductus (vas) deferens smooth muscle pseudostratified columnar epithelium stereocilia sperm in lumen T.S. OF epididymis connective tissue

26. epididymis

27. epididymis stereocilia

28. ductus deferens / (vas deferens) muscle coat

29. PROSTATE

30. fibromuscula septa Prostatic follicle / gland Smooth muscle Simple Columnar epithelium amyloid body or corpora amylacea PROSTATE

31. ureter

32. ureter

33. Figure 21-17 T.S. OF PENIS

34. T.S. OF PENIS

35. seminal vesicle Location on posterior surface of bladder In cut section exhibits honey comb like pattern Seminal vesicles

36. Seminal vesiscle

37. The end

38. Deep to lamina propria there is basal lamina

39. MALE REPRODUCTIVE SYSTEM SERTOLI CELLS SPERMATOGONIA 1º SPERMATOCYTE 2º SPERMATOCYTE SPERMATIDS  SPERMATOGENESIS SPERMATOGONIA 1º SPERMATOCYTE 2º SPERMATOCYTE SPERMATIDS SERTOLI CELLS: - columnar with adjoining lateral processes - Sertoli-Sertoli junctions divide seminiferous tubules into basal and adluminal compartments - extend from basal lamina to lumen

40. Figure 21-5

41. epididymis