Homology Modelling.pptx

protein

1. Homology Modeling (I-TASSER, Phyre 2 ) Presented to: Dr Afshan Kaleem Presented by: Wafa Gulzar

2. Homology Modeling: Homology Modeling refers to constructing an atomic resolution model of the target protein from its amino acid sequence and an experimental three dimensional structure of a related homologous protein, the template .

3. Steps: Find Template Align Sequences Build Model Evaluate Model

4. I-TASSER Is a server that provides an Internet-based service for protein structure prediction. It was found to be one of the best methods in the servers section of CASP experiments.

5. Results Prediction:

6. Phyre2 This modeling uses various detection tools to generate 3D structures. It has special features like ligand binding prediction and variant analysis among the protein amino acid sequence.

7. References: Muhammed , M. T., & Aki‐ Yalcin , E. (2019). Homology modeling in drug discovery: Overview, current applications, and future perspectives.  Chemical biology & drug design ,  93 (1), 12-20. Kelley, L. A., Mezulis , S., Yates, C. M., Wass , M. N., & Sternberg, M. J. (2015). The Phyre2 web portal for protein modeling, prediction and analysis.  Nature protocols ,  10 (6), 845-858.