How blood collected from donors

How blood collected from donors
For Lab.students

1. Prepared By DrAbdulrazzaq Alagbare

2. Steps How Blood collected from Donors
1-Wear a gloves and prepare
equipment's for performing blood
collection
2-Interview the donor and fill out a
questionnaire
3-Make a finger stick to obtain a
drop of blood to test the
hematocrit
DrAlagbare @hematology

3. Steps How Blood collected from Donors
4-Apply a deflated pressure cuff to the
upper arm about 6 cm above the elbow
5-Raise the pressure to between 60
and 80 mm Hg to enable the veins
to be seen and felt.

6-Select a large well situated vein
for the vein puncture, usually near
the bend of the elbow.
DrAlagbare @hematology

4. Steps How Blood collected from Donors
7-Clean very well the required part of
the arm with cotton wool and 70%
ethanol (alcohol).or iodine
8-Wipe dry with a clean swab of
cotton wool.
DrAlagbare
@hematology

5. Steps How Blood collected from Donors
9-Take a blood collecting pack:
10-Make a loose knot (without kinks) in the tubing. When sealing
clips are available, there is no need to make a knot in the tubing.
11-Suspend the bag on a stand about 30 cm below the level of the
donor’s arm.
12-Clamp the tubing near to the needle guard.
13-Whenever possible use plastic forceps to avoid damaging the
tubing.
14-Make a vein puncture with the needle directed upwards in the
line of the vein DrAlagbare
@hematology

6. Steps How Blood collected from Donors
15-Unclamp the tubing to allow the
blood to flow. If necessary,
16-Secure the needle in place with a
small strip of adhesive tape.
17-When the blood begins to flow,
reduce the pressure of the cuff to 40–
60 mm Hg, and ask the donor to
squeeze slowly a small object, as seen
in the photo
DrAlagbare
@hematology

7. Steps How Blood collected from Donors
18-When the blood enters the pack, gently mix it with the
anticoagulant by lifting and tilting the bag.
19-Do not squeeze the bag because this can damage the red cells.
20-Mix the blood a further three times during the donation and
when the donation is finished.
21-Reduce the pressure in the pressure cuff to zero and remove
the object from the donor’s hand.
DrAlagbare
@hematology

8. Steps How Blood collected from Donors
22-Clamp off the tubing 10–15
cm from the needle (see fig).
23-Tie tightly the loose knot in
the tubing or fold back the
tubing and apply a sealing clip,
about 20 cm from the needle
(see fig.).
24-Cut the tubing between the
clamp and knot (or sealing clip)
as shown in following (see fig)
@hematology
DrAlagbare

9. Steps How Blood collected from Donors
25-Collect a blood sample from the
donor into a plain tube for testing
(grouping, HIV screening, etc).
@hematology
DrAlagbare

10. Steps How Blood collected from Donors
31-Dispose of the needle safely.
@hematology
DrAlagbare
32-Mix the blood in the bag and push (‘strip’) the non-
anticoagulated blood from the tubing into the blood bag.
33-Mix and allow the tubing to refill with the anticoagulated
blood.
34-Write clearly the identity number of the donor on the blood
pack and sample tube(s).

11. Steps How Blood collected from Donors
@hematology
DrAlagbare
35-When a blood pack has already been given an identity
number, e.g. from the blood transfusion service use this
number on the sample tube(s).
36-After making sure the bleeding has stopped, cover the
vein puncture site with a pad of cotton wool and adhesive
tape (advise the donor to remove the dressing the
following day).

12. Steps How Blood collected from Donors
@hematology
DrAlagbare
37-Thank the person for donating.
38-Give the donor a drink to make up his or her fluid loss.

13. Steps How Blood collected from Donors
@hematology
DrAlagbare
39-Provide the donor with a dated
certificate of blood donation and
information concerning future
donation.
40-Check that the blood pack and
blood sample(s) have been labeled
correctly and the same identity
number is written on the blood
donation certificate

14. Steps How Blood collected from Donors
@hematology
DrAlagbare
41-Refrigerate the blood after
allowing time for it to cool and for
natural bactericidal activity of white
cells (1–2 h).

15. Steps How Blood collected from Donors
@hematology
DrAlagbare
42-Before refrigerating the blood, take a
1–2 ml sample from the pilot tubing of
the blood pack into a sterile tube.
43-Label the tube with the same
identity number as the blood pack.
44-Store this blood sample in a
separate refrigerator and use it for
compatibility testing to avoid
removing the blood pack from the
blood bank refrigerator

16. END of THE SHOW
@hematology
DrAlagbare