Inhibition of allogenic transplant rejection.Nittal.pptx

Inhibition of allogenic transplant rejection

1. Inhibition of allogenic transplant rejection Nittal Vekaria sem 1 st (M.PHARM) KBIPER

2. PURPOSE AND RATIONALE Transplantation of allogenic organs to recipients results in rejection of the transplants. This effect can be suppressed or delayed by immunosuppressive agents. Various organs are used for allogenic transplantation in animal experiments, such as skin pieces ( Schorlemmer et al. 1993), kidney (Lee 1967; Küchle et al. 1991), rat heart, rat small intestine (Xiao et al. 1944) and corneal buttons (Coupland et al. 1994). The immunosuppressive activity can be evaluated either by using a major histocompatibility complex variant strain combination or a strong allogenic system.

3. Procedure: For skin transplantation male animals of inbred strains of Fischer (F334), Lewis (LEW), Brown Norway (BN), Dark Agouti (DA) rats are used. Rat tail skin (donor) is cut into square pieces of 0.5 to 1.0 cm and transplanted to the tails of recipient rats. Rejection is defined as the day when the skin graft is of red-brown color and hard consistency. As strain combination with a major histocompatibility variant, transplantation from LEW to F334 is performed. Using a strong allogenic system, the high responder DA to LEW donor recipient combination is used. The immunosuppressive agents, e.g., cyclosporine or leflunomide are given orally up to 20 days. Ten animals are used for each group

4. EVALUATION The mean values of rejection time of treated groups are compared statistically with vehicle treated controls using Student’s t-test or the Mann Whitney U-test

5. Refrences : VOGEL HG,EDITOR DRUG DISCOVERY and evaluation:pharmacological assays. Springer science & business media; 2002 jun 13.

6. Thank you….