Instrumentation of UV- Visible Spectroscopy.pptx

Instrumentation of UV- Visible Spectroscopy. pptx

1. A SEMINAR ON Topic: Instrumentation of UV- Visible Spectroscopy Presented By: Hariom Jaiswal M. Pharm (Pharmaceutics) 1 st Year / 1 st Sem (2021-22) INSTTITUTE OF TECHNOLOGY OF MANAGEMENT GIDA, GORAKHAPUR, U.P

2. CONTENTS: INTRODUCTION INSTRUEMENTION APLLICATION

3. INTRODUCTION OF UV- Visible Spectroscopy Ultraviolet and visible spectroscopy is the measurement of the attenuation of the beam of light after it passes through a sample or after reflection form a sample surface absorption measurement can be at single wave length or over an extended spectral range.

4. INSTRUMENTATION OF UV SPECTOSCOPY Instrumentations for measuring the absorption of UV or visible radiation are made up the following components. Light Sources. Filter or Monochromator. Sample containers or Sample cells. Detector.

5. LIGHT SOURCE: The best source of light is the one which is more stable more intense and which gives range of spectorism form 180 to 360 nm . The most commonly used source as Hydrogen or Deuterism lamp. The Xenon discharge lamps and mercury. Various type of radiation source are used. Hydrogen discharge lamp. Deuterism lamp. Xenon discharge lamp.

6. Filter or Mono-chromometer: It is a device used to isolate the radiation of the wavelength from wavelength of the continuous spectra. These are types of monochromatic devices. Filter Paper. Purism. Gratings. It is device are used to isolated the radation of desired wavelength.

7. SAMPLE CONTANIER OR SAMPLE CELLS : The cells or cuvettes are used for handling liquid samples. The cells may either be rectangular or cylindrical in nature and cleaning is carried out washing with distilled water or with dilute alcohol, acetone. The cell may either by cylindercal or rectanguler in nature.

8. DETECTOR: Device which converts light energy into electrical signals, that are displayed on readout devices. Types of detectors: 1- Barrier layer cell/photovoltaic cell. 2-Phototubes/photo emissive cell. 3-Photomultiplier tube.

9. APPLICATION OF UV- SPECTROSCOPY: Qualitative and Quantitative analysis. Detection of Impurities. Structure of elucidation of Organic compound. Structural analysis of Organic compound. Effect of conjugation. Effect of geometric isomerism.