IntAct Database.pptx

protein

1. IntAct DATABASE Submitted To: Dr. Afshan Kaleem Submitted By: Samra Javed Session : 2022-Onwards Lahore College For Women University, Lahore

2. Molecular Interaction Databases At EMBL-EBI

3. IntAct IntAct  is a central, public repository where molecular interactions data can be stored and accessed. It is hosted by the European Bioinformatics Institute (EMBL-EBI) in Hinxton, UK, where it is maintained by a group of curators and developers. IntAct is populated with interaction data from literature curation or direct user submissions. Most of the data refer to protein-protein interactions, but interactions involving other types of molecules, such as small chemical compounds or nucleic acids, can also be found in IntAct. At EMBL-EBI IntAct is the main database for molecular interactions.

4. IntAct IntAct is a member of the International Molecular Exchange ( IMEx ) Consortium a group of major public interaction data providers whose goal is to share curation effort and exchange completed records on molecular interaction data. When you query data in IntAct you also access over 11 million interactions in around 30 data resources via our  PSICQUIC  (Proteomics Standard Initiative Common QUery InterfaCe ) service or a consistently annotated, non-redundant, experimentally determined subset from the IMEx Consortium.

5. Current Status Binary Interactions 1,194,594 Interactions 750,480 Interactors 118,924 Proteins 107,626 Mutation Features 77,348 Experiments 73,943 Publications 22,954 Nucleic Acids 4,639

6. IntAct Search the URL link https://www.ebi.ac.uk/legacy-intact/ . Homepage will be opened.

7. Search Your Protein Enter your protein ID from UniProt e.g. COL2A1 (P02458) and search. Details on same page View and details on separate page Uniprot KB view

8. Human readable form on excel Entire data interaction XGMML Cytosape (Network analysis)

9. Search Goggle H omepage https://www.ebi.ac.uk/legacy-intact/ . Protein Your Search Protein from UniProt e.g. COL2A1 (P02458) Select Customized View Select Standard and Update Select Interactors Select the desired interactor’s box Select S earch I nteractors Result more binary interactions Again Click Interactors Select Protein Show all the proteins involved in interactions Select Compounds Show a list of compound’s details but here is zero Select Nucleic Acids Show list of nucleic a cids but here is zero Select Genes Show a list of gene interactors but here is zero Summary

10. References IntAct (bio.tools). http://www.ebi.ac.uk/intact/.