Intricate regulatory circuit between FLI1 and GATA1/GATA2/LDB1/ERG dictates erythroid vs. megakaryocytic differentiation.

Evaluate an intricate regulatory circuit between FLI1 and GATA1/GATA2/LDB1/ERG dictates erythroid vs. megakaryocytic differentiation.

1. Isabella Santos and Sara Sánchez.
Molecular biology.
Third semester.
UPB.

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FLI1.
(Friend leukemia integration 1)
Transcription factor that inhibits
erythroid differentiation while
promoting megakaryocytic
differentiation by regulating the
expression of GATA1 and GATA2,
among other factors
LDB1.
(LIM domain binding 1)
Scaffold protein that interacts with
various transcription factors,
including FLI1 and GATA1. It plays a
significant role in the regulation of
gene expression during hematopoietic
differentiation.
GATA1.
(GATA binding protein 1)
Transcription factor essential for
erythropoiesis and
megakaryopoiesis. It is involved in
the regulation of genes necessary
for the development of red blood
cells and megakaryocytes.
ERG.
(ETS transcription factor ERG)
Transcription factor that negatively
regulates GATA2 expression. Its
overexpression can inhibit
megakaryocytic differentiation.
GATA2.
(GATA binding protein 2)
Transcription factor that is crucal
for megakaryocytic differentiation.
Is promoted by FLI1.
INTRODUCTION.

3. INTRODUCTION.

4. GENERAL
OBJECTIVE.
Evaluate an intricate regulatory circuit
between FLI1 and GATA1/GATA2/LDB1/ERG
dictates erythroid vs. megakaryocytic
differentiation.

5. 01
Chromatin Immunoprecipitation (ChIP)
The ChIP method is used to analyze the interaction between
proteins and DNA in living cells.
The study suggests that FLI1 regulates the expression of
GATA2 during the differentiation of hematopoietic
progenitors.
METHODS.

6. 02
RT-qPCR
The method quantifies gene expression by measuring the
amount of messenger RNA (mRNA) in a sample.
The results showed that the decrease of FLI1 increased the
expression of GATA1 and decreased that of GATA2, indicating a
regulatory role of FLI1 in cell differentiation.
METHODS.

7. 03
Western Blotting
The Western blot is a technique that allows the detection and
quantification of specific proteins in a sample through separation
by electrophoresis and transfer to a membrane.
This helped validate the results obtained by RT-qPCR, providing a
complete picture of protein regulation during differentiation.
METHODS.

8. 04
METHODS.
Flow Cytometry
Flow cytometry is a technique that allows for the analysis of the physical
and chemical characteristics of cells in suspension.
It is used to study cellular heterogeneity and the expression of markers.

9. RESULTS
Figure 1.

10. RESULTS
Figure 2.

11. RESULTS
Figure 3.

12. RESULTS
Figure 6.

13. AUTHOR. OPINION. SUPPORE OR REFUSE.
Liu F ETAL.
The association between FLI1 and
GATA2 was previously observed,
but it is not known whether this
regulation is direct or indirect.
Ikonomi P ETAL.
Overexpression of GATA2 in these
cells induces megakaryocytic
differentia‑ tion and blocks
erythroid maturation.
Ben‑David Y ETAL.
FLI1 homologue gene ERG is also
implicated in both hematopoietic
stem cell expansion and
hematopoiesis.
DISCUSSION

14. Conclusion 01
The intricate regulatory interactions between transcription
factors like FLI1, GATA1, and GATA2 highlight the necessity of
molecular biology in understanding cell differentiation
processes, which is crucial for developing targeted therapies in
hematological diseases.
Conclusion 03
Identifying specific molecular targets involved in erythroid and
megakaryocytic differentiation opens new avenues for
therapeutic interventions, demonstrating the potential of
molecular biology to address and treat various blood disorders
effectively.
Conclusions
Conclusion 02
Techniques from molecular biology, such as chromatin
immunoprecipitation and RT-qPCR, are essential for elucidating
the complex mechanisms governing gene expression, providing
insights that can lead to innovative medical treatments.

15. Isabella santos´concept map.

16. by: Sara Sánchez Valencia