Intrinsic in Situ Bioremediation

P.Pandiyammal @ Subathra
III B.sc Biotechnology
Ayya Nadar Janaki Ammal College,
Sivakasi, Tamilnadu.

1. INTRINSIC IN SITU BIOREMEDIATION P . Pandiyammal @ Subathra 18UT12 III B.sc Biotechnology

2. BIOREMEDIATION Bioremediation is the use of living organisms, primarily microorganisms, for the degradation of hazardous chemicals in soil sediments, water, or other contaminated materials into less toxic forms Microorganisms metabolize the chemicals to produce carbon dioxide or methane, water and biomass E nzymatically transformed to metabolites that are less toxic or innocuous I n some instances, the metabolites formed are more toxic than the parent compound. For example, perchloroethylene and trichloroethylene may degrade to vinyl chloride .

3. IN SITU BIOREMEDIATION: In situ bioremediation involves a direct approach for the microbial degradation of pollution. In situ bioremediation is when the contaminated site is cleaned up exactly where it occurred . Biostimulation : Addition of adequate quantities of nutrients at the site promote microbial growth is done. When microorganisms are imported to a contaminated site to enhance degradation, the process is called as Bio-augmentation There is no need to excavate or remove soils or water in order to complete remediation .

4. INTRINSIC BIOREMEDIATION Intrinsic bioremediation uses micro organisms already present in the environment to biodegrade harmful contaminant. There is no human intervension involved. Most commonly used The cheapest means of bioremediation available

5. The intrinsic that is inherent capacity of microorganisms to metabolize the contaminants should be tested at the laboratory and field levels before use for intrinsic bioremediation. Through site monitoring programmes progress of intrinsic bioremediation should be recorded time to time. The conditions of site that favour intrinsic bioremediation are ground water flow throughout the year, carbonate minerals to buffer acidity produced during biodegradation, supply of electron acceptors and nutrients for microbial growth and absence of toxic compounds. Bioremediation of waste mixture containing metals such as Hg, Pb , and cyanide at toxic concentration can create problem

6. Factors effecting rate of intrinsic bioremediation Moisture in soil Ph Temperature Soil nutirents Presence of electron acceptors Toxin concentration

7. ADVANTAGES OF IN SITU BIOREMEDIATION Cost effective, with minimal exposure to public or site personnel Sites of bioremediation remain minimally disrupted DISADVANTAGES OF IN SITU BIOREMEDIATION Very time consuming process Sites are directly exposed to environmental factors such as temperature, oxygen supply etc . Microbial degrading ability varies seasonally

8. Thank you