Introduction to Histotechnology.pptx....

Introduction to Histotechnology.pptx....

1. Introduction to Histotechnology Dr. Jagroop Sidhu PhD Biochemistry Govt. Medical College Amritsar

2. What is Histotechnology? D Introduction to Histotechnology 2 Definition: Histotechnology is the science of preparing and analyzing tissues to study the microscopic structure of cells and tissues. Importance: Essential for diagnosing diseases, understanding tissue structure, and conducting research.

3. Applications of Histotechnology Presentation title 3 Medical Diagnosis: Pathology, cancer diagnosis, infectious diseases. Research: Cell biology, developmental biology, pharmacology. Forensics: Identifying tissue samples in forensic investigations.

4. The Histotechnology Process Presentation title 4 Specimen Collection Fixation Processing Embedding Sectioning Staining Microscopic Analysis

5. Specimen Collection Introduction to Histotechnology 5 Types of Specimens: Biopsies, surgical resections, autopsies. Handling Techniques: Proper labeling, avoiding contamination, timely transfer to the lab.

6. Fixation Purpose: Preserve tissue structure and prevent degradation. Common Fixatives: Formaldehyde, paraformaldehyde, glutaraldehyde. Process: Immersing tissue in fixative for a specified time. “ Dr. Sidhu ”

7. Tissue Processing Dehydration: Removing water using alcohol. Clearing: Replacing alcohol with a clearing agent like xylene. Infiltration: Embedding tissue in paraffin wax.

8. Embedding Purpose: Provide support for thin sectioning. Techniques: Using molds to embed tissue in paraffin blocks .

9. Sectioning Microtomy: Cutting thin sections (3-5 microns) using a microtome. Cryotomy : Cutting frozen sections for rapid diagnosis.

10. Staining Purpose: Differentiate tissue components. Common Stains: Hematoxylin and eosin (H&E), special stains (PAS, Masson's trichrome). Immunohistochemistry: Using antibodies to detect specific proteins.

11. Microscopic Analysis Types of Microscopes: Light microscope, fluorescence microscope, electron microscope. Analysis Techniques: Identifying cellular structures, and comparing normal vs. pathological tissues.

12. Histotechnology Lab Setup Equipment: Microtomes, embedding centers, staining machines, microscopes. Safety: Proper handling of chemicals, use of PPE, waste disposal.

13. Careers in Histotechnology Job Roles: Histotechnician , histotechnologist, pathology assistant, research scientist. Qualifications: Certification, degree in histotechnology or related field. Challenges and Future Directions Challenges: Quality control, standardization, evolving techniques. Future Trends: Automation, digital pathology, advanced staining techniques.

14. Thank you Dr. Jagroop Sidhu​ [email protected]