Invertoscope or Inverted microscope - Pathology - MLT

Topic: Invertoscope or Inverted microscope
Faculty: Pathology
Course: BSc MLT - 3rd year

1. INVERTED MICROSCOPE Dr. Salman Ansari Dept. of Pathology Kanachur Institute of Medical Sciences

2. Contents Inverted microscope Principle Parts Working Applications Advantages Disadvantages

3. Inverted Microscope(Invertoscope) An inverted microscope is a microscope with its light source and condenser on the top above the stage, while the objectives and turret are below the stage Invented in 1850 by American chemist - J. Lawrence Smith Used for observation of biological specimens Also called “cell culture microscope”

4. Principle of inverted microscope Same as that of compound microscope T he source for transmitted light and the condenser are placed on the top of the stage, pointing down toward the stage The objectives are located below the stage pointing up The specimens or cells are observed through the bottom of the cell culture vessel

5. Parts of an invertoscope Light source Condenser Stage: fixed stage - large, to hold vessels like petri dish Objective lens: movable lenses of different magnifications Turret(nosepiece): holds the lenses Eyepiece Knobs: for fine and coarse adjustment

6. CONDENSER LIGHT SOURCE EYE PIECE OBJECTIVE PARTS OF AN UPRIGHT MICROSCOPE

7. PARTS OF AN INVERTED MICROSCOPE

8. A - Light source B - Condenser C - Sample dish D - Stage E - Objective lens F - Eyepiece Simplified diagram

9. Working Place the specimen in a slide or glass container on the stage. The stage does not move at all. Set the focus by moving the condenser and the objective lenses using the adjusting knob. The specimen can be observed through the ocular lens

10. Applications It can be used to observe the living cells in their natural state present at the bottom of any glass container such as a Petri plate, flask, etc. without preparing the slide also used in diagnostic assays such as microscopic observation drug susceptibility(MODS) assay for TB useful in fungal cultures e.g. detection of Phytophthora species in cultures. It is also helpful in parasitology to observe nematodes such as Vermiform nematodes .

11. Advantages It has a wide stage that favors it to view specimens in Petri plates and therefore, it is commonly used to study live cells , by viewing the cells from the bottom of the cell culture apparatus. It can be used to view the cell tissues in their original vessel , which are larger than microscopic slides, which makes it better than the upright microscope which only views specimens in small microscopic slides. It can be used to view cells in large quantities of the medium than in small specimen quantities on a glass slide under a coverslip. No contact between sample and objective, hence sterility is maintained

12. Limitations Very costly Manufactured by only few companies It is difficult viewing the specimens through thick glass vessels such as a Petri plate hence they require very high optical quality.

13. Summary Upright vs inverted microscope Principle? Parts? Uses? Advantages? Disadvantages?

14. Name the parts

15. For notes, scan: References: https://microbenotes.com/inverted-microscope/ Questions: [email protected] For PPT, scan: