KIM706_Ders_5_Mass Spectrometry_041223.pdf

KM MS

1. KİM 706
KÜTLE SPEKTROMETRİSİ
04/12/2023
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2. Mass Spectrometry (MS)
Vital tool used to characterize and analyze molecules
•Limitations
•Biomolecules and organic macromolecules are fragile
•Molecular ions or meaningful fragments were limited to only 5-10 kDaat the time
•New technique
•In 1987, Michael Karasand Franz Hillenkampsuccessfully demonstrated the use of a matrix to
ionize high molecular weight compounds.
M. Karas, et al and F. Hillenkamp; International Journal of Mass Spectrometry and Ion Processes, 78; 1987, p53.
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3. Inlet
Ion
source
Mass
Analyzer
Detector
Data
System
High Vacuum System
Mass Spectrometer
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4. •ElectronIonization(EI)
•ChemicalIonization(CI)
•DesorptionChemicalIonization(DCI)
•FieldDesorption(FD)
•FieldIonization(FI)
•FastAtom Bombardment(FAB)
•SecondaryIonMassSpectrometry(SIMS)
•Desorptionelectrosprayionization(DESI)
•Direct Analysis in Real Time(DART)
•AtmosphericPressureChemicalIonization(APCI)
•AtmosphericPressurePhotoionization(APPI)
•ElectrosprayIonization(ESI)
•Matrix-AssistedLaserDesorptionIonization(MALDI)
IONIZATION METHODS
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5. Matrix-Assisted Laser Desorption/Ionization
(MALDI)
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6. MALDI Matrix
•Be able to embed and isolate analytes(e.g. by co-
crystallization)
•Be soluble in solvents compatible with analyte
•Be vacuum stable
•Absorb the laser wavelength
•Cause co-desorption of the analyteupon laser irradiation
•Promote analyteionization
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MALDI Matrix

8. MALDI matrix
Matrix
1,8,9-Trihydroxyanthracen
(Dithranol) polymers
2,5-Dihydroxybenzoic acid
(DHB)
proteins, peptides, polymers
-Cyano-4-hydroxycinnamic
acid
peptides, (polymers)
4-Hydroxypicolinic acid oligonucleotides
Trans-Indol-3-acrylacid
(IAA)
polymersOHOHOH OH
OH
COOH CCH
OH
CN COOH N
OH
COOH N
H
COOH
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9. Sample Preparation: DriedDroplet
solved Matrix solved sample
Mixing and Drying
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Applications of MALDI mass spectrometry
•Peptides and proteins
•Synthetic polymers
•Oligonucleotides
•Oligosaccharides
•Lipids
•Inorganics
•Bacterial identification
•Imaging**
…
•Used especially
•Proteomics

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12. MALDI IMAGING
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FARE BEYİN DOKUSU
CLOZAPINE

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MALDI-MSI imagesof ratkidney(45 µm pixelsize, unpublisheddata). Non-negativematrixfactorisation(NMF),
performedafterisolationof tissueregionbyprincipalcomponentsanalysis(PCA), isolatestherenalmedulla
(green, factor2), renalcortex(magenta, factor3), renalpelvis(blue, factor4) andvasculature(red, factor10).
http://www.npl.co.uk/science-technology/surface-and-nanoanalysis/research/matrix-assisted-laser-desorption-ionisation-maldi

15. MALDI IMAGING
15 1821823_Bruker_MALDI_Imaging_brochure_07-2013

16. MALDI IMAGING
16 1821823_Bruker_MALDI_Imaging_brochure_07-2013

17. 17 1821823_Bruker_MALDI_Imaging_brochure_07-2013

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