Lab 2- B443 -2025.hhhhhhhhooooooormonepdf

Hormone

1. Plant hormone and Tissue culture
B 443
Practical session 2
Practical book pp. 13-24

3. This Photo by Unknown Author is licensed under CC BY-SA-NC

5. Our Run
•1: IAA
•2: GA3
•3: KIN
•4: Mix
•5: sample
2.5 cm
1 2 3 4 5

8. Go to Run
This Photo by Unknown Author is licensed under CC BY-SA-NC

9. After Run
•Leave the paper to dry
•Then directly exam under UV Lamp (254/360 nm) to detect the different
hormones. e.gIAA reveal as fluorescent.

14. Detection and identification of hormones chemically

15. Purple spot
Pink spot
R
f = 0.45

16. Green spot
R
f = 0.4

17. Dark green
R
f = 0.89

18. Detection of hormones by UV absorption spectra . Readings :
 IAA ABA
220 0.5874 0.925
230 0.6942 1.092
240 0.4776 1.297
250 0.4470 1.441
260 0.6909 1.490
270 2.109 1.391
280 2.48 0.993
290 1.579 0.454
300 1.823 0.160
310 0.150 0.035
320 0.039 0.020
330 0.036 0.010
340 0.023 0.020
350 0.014 0.008

19. •Illustrate figures and Comment:
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20. Trial : spotting on TLC (thin layer
chromatography)
•Repeat as in case of paper chromatography however the running
solvent is :
•Methanol : Water
•80:20