Lab 3 – actinomycosis & Rhinoscleroma without audio.pdf

practical lab 3 Lab 3 – actinomycosis & Rhinoscleroma without audio

1. Lab 3 –actinomycosis&
Rhinoscleroma
Dr. NaglaaAli Ramadan
Professor of Pathology
Faculty of medicine –ZagazigUniversity

2. Actinomycosis
•Definition:
•Actinomycosis is a chronic suppurative
granulomatous diseasecaused by
gram positive, branching, filamentous
anerobicbacteria “Actinomyces
israelii”which normally reside in
human oropharynx, GIT and vagina

3. Actinmycosis
•Pathology:
•Microscopic features:
1-Bacterial colonies (sulphur granules):consist of peripherally arranged red stained clubs and centrally interlacing gram positive branching filaments which are stained deep blue.
2-Inflammatory cellssurrounding the colonies. These consist of neutrophils, pus cells,macrophages, and lymphocytes.
3-Fibrous tissueat the periphery.

4. Actinomycosis
bacterial

8. Sulphur granule
within an abscess
Individual filaments of A. israeli
visible with silver impregnation
technique
Actinomycosis

9. Rhinoscleroma
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Klebsiellarhinoscleromatis

10. Rhinoscleroma
•Microscopic picture:
•Section in a granuloma shows:
•Mickulickzcell: the predominant cells, large rounded
with foamy cytoplasm and small darkly stained central
nuclei. (macrophages with hydropicdegeneration).
Miculiczcells

11. Rhinoscleroma
•Russelbodies: few in number, rounded or oval,
refractilestructurelessand red stained with eosin. Some
of the cells show flattened nuclei at the periphery , others
are devoid of nuclei ( plasma cells with hyaline
degeneration).
•Scattered lymphocytes, plasma cells, collagen bundles
and blood vessels.

12. Russelbodies
Miculiczcells

13. Rhinoscleroma

14. Rhinoscleroma
nx100

15. Rhinoscleroma

16. Rhinoscleroma

17. Rhinoscleroma
nx400
Russel bodies