lab safety and instrumental analysis.pptx

instrumental analysis

1. Lab 1 Instrumental analysis

2. I- Safety procedures A-Chemicals B- Ultraviolet light C- Electricity D- General housekeeping II- Equipment III- sterile technique

3. Lab safety and equipment I- Safety procedures: A-Chemicals: 1- A number of chemical used in any molecular lab are hazardous 2- material safety data sheet (MSDS) 3- This information contains *chemical name *Health hazard data *Physical data *Fire and explosion data *Reactivity data *Precaution needed when handling this chemical. This MSDS should be kept in lab. You must read this information prior using a new chemical.  

4. Example: * Phenol - cause sever burns * Acrylamide - a potential neurotoxin * Ethidium bromide - carcinogen. These chemicals are not harmful if used properly.   Precautions: 1-wear gloves 2-never mouth pipette them 3- Immediately clean the skin when any of these chemicals spill on it 4- Discard the waste in appropriate container.

5. Disposal of buffers and chemicals   1- Uncontaminated agar or gel should be discarding in the trash not in the sink and the bottles rinsed well 2- Contaminated media and Petri dishes should be autoclaved before discard. 3- Organic reagents such as phenol and ethidium bromide should be disposed in labeled container not in sink or trash.

6. B- Ultraviolet light Exposure to UV light can cause severe eye damage , therefore use appropriate eye protection when using UV lamb.  C- Electricity The voltage used in electrophoresis is sufficient to cause electrocution. Precaution: * cover the buffer reservoirs during electrophoresis * Turn of the power supply before removing the gel. D- General housekeeping * Keep your area clean * Every thing stored in incubator, refrigerator…. etc should be labeled.

7. II- Equipment 1- Micropipette accurately measuring and transport volumes of solutions.   2- PH meter measure potential difference between reference electrode and glass electrode. Accurate pH reading depends on standardization The sample to be PHed should be at room temperature and stirring gently on a stir plate.

8. 3- Autoclave:  

9. 4- Spectrophotometer:

10. 5- PCR (polymerase chain reaction) device analysis of any short sequence of DNA even in samples containing minute quantities of DNA.   6- Oven   for heating or drying objects   7- Water bath   regulating temperatures of any subjects to heat   8- centrifuge Used for isolating and separating suspensions and immiscible liquids. It varies widely in speed, capacity and temperature control

11. 9-Vortex:  

12. 10- gel electrophoresis