lethal genes

gene interaction

1. LETHAL GENES
By
Dr. Priti D.Diwan
Assistant Professor
Department of Zoology
J.D.Patil Sangludkar Mahavidyalay Daryapur.

2. LETHAL GENES
Genes which result in the reduction
of viability of an individual or become
a cause for death of individuals
carrying them are called as lethal
genes.
Certain genes are absolutely
essential for survival. Mutation in
these genes creates lethal allele.

3. Lethal genes were first
discovered by Lucien Cuénot while
studying the inheritance of coat
colour in mice.
He expected a phenotype ratio
from a cross of 3 yellow:1 white,
but the observed ratio was 2:1.

4. TYPES OF LETHAL ALLELES
Lethal alleles fall into four categories.
1. Early onset-lethal alleles which
result in death of an organism at
early stage of life, for example,
during embryogenesis.
2. Late onset-lethal allele which kills
organism at their final stage of life
are known as late onset allele.

5. 3. Conditional-lethal allele which kill an
organism under certain environmental
conditions only.
e.g., some temperature sensitive alleles
kill organisms only at high temperature.
4. Semi lethal –Lethal allele which kill only
some individuals of the population but
not all are know as semi lethal.

6. Lethal alleles are dominant or
recessive.
Fully dominant lethal allele kills
organism in both homozygous and
heterozygous condition.
Recessive lethal alleles kills organisms
in homozygouscondition only.

7. DOMINANT LETHAL GENES
•Dominant lethal allele kills both in
homozygous and heterozygous states.
•Individuals with a dominant lethal allele die
before they can leave progeny.
•Therefore, the mutant dominant lethal is
removed from the population in the same
generation in which it arose.

8. EXAMPLESOFDOMINANTLETHALALLELES
•An example is the "creeper" allele in chickens, which
causes the legs to be short and stunted.
•Creeper is a dominant gene, heterozygous chickens
display the creeper phenotype.
•If two creeper chickens are crossed, one would
expect to have (from Mendelian genetics) 3/4 of the
offspring to be creeper and 1/4 to be normal.
•Instead the ratio obtained is 2/3 creeper and 1/3
normal.
•This occurs because homozygous creeper chickens
die.

10. HUNTINGTON’S DISEASE
•Huntington disease is a progressive brain
disorder that causes uncontrolled
movements, emotional problems, and
loss of thinking ability (cognition).
•Adult-onset Huntington disease, the most
common form of this disorder, usually
appears in a person's thirties or forties.

11. •Mutations in the HTT gene cause
Huntington disease. The HTT gene
provides instructions for making a
protein called huntingtin.
•HH Individual dies of Huntington’s
disease
•Hh Individual dies of Huntington’s
disease
•hh Normal individual

12. •Early signs and symptoms can include
irritability, depression, small involuntary
movements, poor coordination, and trouble
learning new information or making decisions.
•Affected individuals may have trouble walking,
speaking, and swallowing.
•Individuals with the adult-onset form of
Huntington disease usually live about 15 to 20
years after signs and symptoms begin.

14. RECESSIVE LETHAL GENES
Recessive lethal genes kill only when
they are in homozygousstate. They may
be of two kinds:
•one which has no obvious phenotypic
effect in heterozygotes.
•one which exhibits a distinctive
phenotype when heterozygous.

17. EXAMPLES OF RECESSIVE
LETHAL
ALLELLES•Brachydactyly –
A genetic state in which the fingers are unusually
short in heterozygotic condition. But, this
condition is lethal during early years to
homozygous recessive individuals due to major
skeletal defects.
Most surgeries for brachydactyly are cosmetic.
Some therapy might be needed to help with
kinesthetic activities.

28. What causes Brachydactyly?
•A mutation occurs in IHH gene which encodes
proteins responsible for bone growth and
differentiation.
•When a single mutated copy of the allele is present,
the phenotype has just few deformations of skeletal
bones. This is because one dose of functional IHH
allele is almost enough to produce a required amount
of a protein essential for a skeletal formation.
•If an organism inherits two mutated copies of IHH
allele no protein essential for skeletal bones
formation is produced and development of embryo
cannot be continued -the embryo dies.

29. Let's say that an allele a is recessive and codes
for a completely dysfunctional form of a
protein essential for bone growth, and A is a
dominant wild type allele. If heterozygotes for
these alleles procreate, then:

31. Sickle Cell Anemia –
•A genetic state that is often fatal in the
homozygous recessive condition.
•People who inherit one good copy of the gene
and one mutated copy are carriers. They are
clinically normal, but can still pass the
defective gene to their children.
•When sickle-shaped red blood cells get stuck
in blood vessels, patients can have episodes of
pain called crises. Other symptoms include
delayed growth, strokes, and jaundice
(yellowish skin and eyes because of liver
damage).

32. Genetics of Sickle Cell Anemia
Genotypes
Hb
N
Hb
N
Hb
N
Hb
S
Phenotypes
Normal haemoglobin
Sickle cell trait
Hb
S
Hb
S
Sickle cell anaemia
Animation showing sickle cells

35. Cystic Fibrosis
•A genetic state that is fatal to every
homozygous recessive person by age 30.
•Sticky mucus accumulates in the lungs
giving rise to constant and risky
respiratory infections.
•It is caused due to malfunctioning of
chloride ion channels in ducts.

36. Lungs in cystic fibrosis
Normal lung CF lung
Normal alveolar appearance
Dilated crypts
filled with mucus and bacteria.

37. Congenital Ichthyosis
•Children with this disease are born with
crusted leathery skin with deep splits.
•These splits lead to bleeding, infection and
death.
•In Ichthyosis, the skin's natural shedding
process is slowed or inhibited and in some
types, skin cells are produced too rapidly.
•Most types of autosomal recessive congenital
ichthyosis require two forms of treatment -a
reduction in the amount of scale buildup and
moisturising of the underlying skin.

39. COAT COLOUR IN MICE
•The coat colour of mice is governed by a
multiple allelic series in which A allele
determines agouti, A
Y
allele determines yellow
coat and a allele forms black coat.
•The dominance hierarchy is as follows:
A
Y
> A > a
The A
Y
allele acts as recessive lethal, since in
the homozygous state (A
Y
A
Y
), it kills the
individual in early embryonic state.

40. Thus, when two
yellow coated
heterozygotes ( A
Y
A)
are crossed, they
produce a progeny
showing a ratio of
2:1 since
homozygous yellow
individuals ( A
Y
A
Y
) are
never born due to
lethal effect of AY
gene.

41. GENE THERAPY

42. Gene therapy is the insertion of
genes into individual cells and
tissues to treat a disease in
which a defective mutant allele
is replaced with a functional
one

43. There are four approaches:
•A normal gene inserted to compensate
for a non-functional gene.
•An abnormal gene expression is
suppressed.
•An abnormal gene repaired through
selective reverse mutation.
•Regulation of particular gene could be
altered(degree to which a gene could be
turned on or off).

44. TYPES OF GENE THERAPY
SOMATIC CELL GENE THERAPY GERM LINE GENE THERAPY
•Therapeutic genes are •Therapeutic genes are
transferred into the
somatic cells.
transferred into the
germ cells.
•Eg: introduction of
genes into the bone
marrow cells, skin cells,
blood cells etc
•It is non heritable and
cannot be passed on to
the next generations.
•Eg : genes introduced
into the eggs and
sperms
•It is heritable and
passed on to the next
generations.

45. SOMATIC CELL THERAPY

46. GERM LINE THERAPY

47. STEPS IN GENE THERAPY
1. Identification of the defective gene.
2. Cloning of normal healthy gene.
3. Identification of target cell / tissue / organ.
4. Insertion of the normal functional gene into the
host DNA
5. Transferred gene (TRANSGENE) encodes &
produces proteins
6. The Proteins encoded by Transgene corrects the
disorder

48. APPROACHES TO
GENE
THERAPY
EX-VIVO IN-VIVO

50. EX-VIVO APPROACH
•Target cells are removed
from the body and grown
in vitro.
•The gene is then
introduced into the
cultured cells.
•These cells are then re-
introduced into the same
individual
•Examples: Fibroblast cells,
Hematopoietic cells.

52. EXAMPLE OF EX-VIVO GENE THERAPY
•1st gene therapy –to correct deficiency of
enzyme, Adenosine deaminase (ADA).
•Performed on a 4yr old girl Ashanti DeSilva.
•Wa s suffering from SCID-Severe Combined
Immunodeficiency.
•Caused due to defect in gene coding for ADA

54. In vivo approach:
STEPS: (Direct Gene Transfer)
1. Cloned
therapeutic gene is
introduced directly
into the affected
tissue, without
removing cells
from the body.
2. Specially designed
vehicles are
needed.
3. Examples are:
Lungs, Brain

55. Video of gene therapy

56. EXAMPLE OF IN VIVO-GENE THERAPY
•In patients with cystic fibrosis, a protein called
cystic fibrosis trans-membrane regulator (CFTR)
is absent due to a gene defect.
•In the absence of CFTR chloride ions concentrate
within the cells and it draws water from
surrounding.
•This leads to the accumulation of sticky mucous
in respiratory tract and lungs.
•Treated by in vivo replacement of defective gene
by adenovirus vector .

58. ROLE OF VECTORS IN GENE THERAPY

59. METHODS OF GENE DELIVERY
•Viral Vectors:
–Adenovirus
–Retrovirus
–Adeno-associated virus (AAV)
–Herpes simplex virus (HSV)
•Non-viral vector based:
–Naked DNA (plasmid DNA): injection or
gene gun
–Liposomes (cationic lipids): mix with genes

60. VIRAL VECTORS

61. ADENOVIRAL VECTORS
•They are double stranded DNA genome.
•Adenoviruses are able to deliver large
DNA particles (up to 38 kb).
•They do not integrate into the host
genome, their gene expression is too
short term.

62. •Advantages
–High titers
–Both dividing and non-dividing cells
–Wide tissue tropism
–Easily modify tissue tropism
•Disadvantages
–Transient expression ( not good for genetic
diseases)
–Highly immunogenic
–High titers of virus can be toxic
–More suitable for cancer immunotherapy

63. RETRO VIRAL VECTOR
•The genetic material in retroviruses is in the form
of RNA molecules.
•They can transfect dividing cells because they can pass
through the nuclear pores of mitotic cells.
•Retroviruses are useful for ex vivo delivery of somatic
cells because of their ability to linearly integrate into
host cell genome.
•Double stranded DNA copies are produced from RNA
genome.
–The retrovirus goes through reverse transcription
using reverse transcriptase and RNA
–the double stranded viral genome integrates into
the human genome using integrase

64. CHARACTERISTICS OF RETRO VIRUS
•Advantages
–Integration: permanent expression
–Good for genetic disorders
•Disadvantages
–Only infecting dividing cells
–Insertional mutagenesis

65. ADENO-ASSOCIATED VIRUS VECTORS
•It is a human virus that can integrate into
chromosome 19.
•It is a single stranded, non pathogenic small
DNA virus.
•A AV enters host cell, becomes double
stranded and gets integrated into
chromosome.

66. CHARACTERISTICS OF AAV VECTOR
•Advantages
–Integration and persistent expression
–No insertional mutagenesis
–Infecting dividing and nondividing cells
–Safe
•Disadvantages
–Size limitation, 4.9 kb
–Low titer of virus, low level of gene
expression

67. HERPES VIRAL VECTORS
•The genome consists of one double-stranded DNA
molecule which is 120 to 200 kilobases in length.
•The virus itself is transmitted by direct contact and
replicates in the skin or mucosal membranes
before infecting cells of the nervous system.
•They allow for a large DNA insert of up to or
greater than 20 kilobases; they have an extremely
high titer.
•They have been shown to express transgenes for a
long period of time in the central nervous system.
•They are far from complete and require much
additional engineering to be as efficient as hoped.

68. WHY USE VIRAL VECTORS?
•They are obligate intracellular parasites
•Very efficient at transferring viral DNA
into host cells
•Specific target cells: depending on the
viral attachment proteins (capsid or
glycoproteins)
•Gene replacement: non-essential genes
of virus are deleted and exogenous genes
are inserted

69. DISADVANTAGES OF VIRUSES AS
VECTOR IN GENE THERAPY
•In all viral types, the vectors tend not to
disperse well in a targeted tissue. Even when
injected directly into a tumor, they are prone
to miss some of the targeted cells.
•In addition, their use does not allow long-
term gene expression.

70. NON-VIRAL VECTORS

71. PHYSICAL APPROACHES
Needle injection
Electroporation
Gene gun
Ultrasound
Hydrodynamic delivery
The simplest method of non-viral transfection.
Clinical trials carried out of intramuscular
injection of a naked DNA plasmid have
occurred with some success; however, the
expression has been very low in comparison
to other methods of transfection.

72. LIPOSOMES
WHY NAKED DNA???
Lets’ wrap it in something safe
to increase transfection rate
Lipids –are an obvious idea !

73. WHAT ARE LIPOSOMES?
•They are artificial vesicles with a phospholipid
bilayer membrane.
•They are self-closing spherical particles where
one or several lipid membranes encapsulate
part of the solvent in which they freely float in
their interior.
•Liposomes are typically 5-10 µm in diameter
with the phosopholipid bilayer about 3 nm
thick

74. Advantages of liposomes
Cheaper than viruses
No immune response
Especially good
for in-lung delivery (cystic
fibrosis)
100-1000 times more plasmid DNA needed
for the same transfer efficiency as for viral vector

75. DNA delivery of genes by liposomes
Fig. 19.20aa (TE Art)
Copyright©TheMcGraw-Hill Companies, Inc.
Permissionrequiredforreproductionordisplay. Fig. 19.20ab (TE Art)
Copyright©TheMcGraw-Hill Companies, Inc.
Permissionrequiredforreproductionordisplay.
Liposome Endosome
DNA carrying the
gene of interest
By recombination, the DNA carrying
the gene of interest is integrated
into
a chromosome of the target cell.Target cell
DNA-liposome complex is taken
into
the target cell by endocytosis.
The liposome is degraded within the
endosome
and the DNA is released into the cytosol.
Integrated
gene
The DNA is imported into the cell
nucleus. Nonviral approach

76. ARTIFICIAL CHROMOSOME
•Another method is trying to introduce a
47th chromosome.
•It exists alongside the 46 others.
•It could carry a lot of information.
•But how to get the big molecule through
membranes?

77. ETHICAL ISSUES
•Who will have access to therapy?
•Is it interfering with God’s plan?
•Should people be allowed to use gene therapy to
enhance basic human traits such as height, intelligence
etc.?
•Is it alright to use the therapy in the prenatal stage of
development in babies?

78. gene