APARNA A 1 st year MSc Nursing College Of Nursing Kottayam LUMBAR PUNCTURE
DEFINITION LUMBAR PUNCTURE or SPINAL TAP is carried out by inserting a needle into Lumbar subarachnoid space to withdraw C S F
To obtain C S F for analysis & diagnosis of: Meningitis Meningoencephalitis Subarachnoid hemorrhage Malignancy – diagnosis and treatment Pseudotumor Cerebri Other neurologic syndromes To drain C S F & reduce intracranial space To instill medications PURPOSES
Increased intracranial pressure Head CT before study if focal neurologic findings present to rule out impending cerebral mass herniation If platelet count is less than 40,000 and Prothrombin time is less than 50% of control CONTRAINDICATIONS
Hydrocephalus- Enlarged ventricle size & in suspected normal pressure Hydrocephalus Coma- If C T is negative and I C P increased Meningitis- Exclude mass lesion & confirm diagnosis L P IN CERTAIN DISEASES
Use smallest possible gauge [20/22] Prefer atraumatic rather than cutting needle Technique 1.5 in for < 1 yr 2.5 in for 1 year to middle childhood 3.5 in for older children and adolescents Larger for large adolescents
Needle is inserted into subarachnoid space through intervertebral space INSERTION OF NEEDLE
Spinal cord ends at L1-L2, so sites for puncture are located at L3-L4 or L4-L5 Restrain patient in lateral decubitus position Maximally flex spine without compromising airway Keep alignment of feet, knees and hips Position head to left if right handed or vice versa POSITION OF PATIENT
Equipment Anesthetic such as: Topical- Zylocaine cream or Lidocaine 1% with 25 gauge needle and syringe Povidone-iodine solution & sponge Drapes, gauze, and bandages Manometer, stopcock, tubing and specimen bottles Sterile CSF tray with Spinal needle
Obtain a written consent for the procedure Explain the procedure to the patient Determine whether patient have any doubts or misconceptions Reassure the patient Instruct patient to void after procedure Pre- Procedure
Procedure Position the patient at one side of edge of bed Place a small pillow under patient’s head & another between the legs Assist the patient to maintain position Encourage patient to relax & to breath normally Describe the procedure step by step as it proceeds
Procedure Contd…. The physician cleanses the site with antiseptic solution and drapes the site Local anesthetic is injected to numb the site and a spinal needle is inserted to subarachnoid space with stylet with bevel up to keep cutting edge parallel with nerve and ligament fibers
A specimen of C S F is collected usually in three test tubes Needle is withdrawn & a small dressing is applied at puncture site Sent specimen to lab immediately Procedure Contd….
Instruct patient to lie on prone for 2 to 3 hours Monitor patient for any complications Encourage increased fluid intake Post-Procedure
Headache Back pain [Occasionally with short-lived ] Disc herniation if needle advanced too far Bleeding or fluid leak around spinal cord Infection, pain, hematoma Subarachnoid epidermal cyst Ocular muscle palsy (1%) Nerve Trauma Brainstem herniation COMPLICATIONS
Throbbing bifrontal & occipital headache Dull and deep in character Severe on sitting or standing IT CAN BE AVOIDED BY: Using small gauge needle Keep patient prone after procedure for 2 hours, then side-lying for 2-3 hours, then supine or prone for 6 or more hours Post Lumbar Puncture Headache
Bed rest Analgesics Hydration Epidural blood patch MANAGEMENT
Clear and colourless Secreted by choroid plexus Exists in subarachnoid space It is about 150-200ml acts as shock absorber transports nutrients C S F ANALYSIS
If C S F is blood tinged 3 samples has to be collected Uniformly stained SA H CSF clears in 3 rd bottle-Traumatic trap 1 2 3 C S F ANALYSIS 1 2 3
Usually obtained for cell count, culture, glucose and protein testing R B C and Differential W B C Bacteriological –Gram stain and culture Biochemical-Protein[0.15-0.45g/l] - glucose [0.45-0.70g/l] STANDARD TESTS
SAH : Spectrophotometry Malignant Tumor : Cytology Tuberculosis : Polymerase chain reaction, Jensen Culture Non-bacterial Infection : Virology, fungal & parasitic studies Demyelinating Disease : Oligoclonal bands Neurosyphilis : V D R L test Cryptococcus : culture, antigen detection H I V : culture, antigen detection & antiviral antibodies SPECIAL TESTS