Magnification and microscopes

microscpes

1. Microscopes!

2. Mutual dependence: Science & Technology Technology…the pragmatist Does it work? Is it useful? Is it sustainable? Science…the idealist What is it? How does it work? What does it tell us?

3. Mutual dependence: Science & Technology Microscopes Electron microscopes Higher resolution Dead samples Expensive Optical (light) microscopes Lower resolution Live samples Less expensive

4. Micrographs Scanning electron micrograph (SEM) of various Pollen. Public domain image reference: Dartmouth Electron Microscope Facility, Dartmouth College

5. Size matters micron = micrometer = 1/1,000,000 meter diameter of human hair = ~20 microns Miss N, Show them the interactive! !

6. Microscopic units of measurement

7. 1 metre (m) = ________ millimetres (mm) 1 mm = ________ microns ( µm) 1 µm = ________ nanometres (nm) 1 mm = ________ nm 1 m = ________ µm 1 m =________ nm

8. 1 m = 1,000 mm = 10 3 mm 1 mm = 1,000 µm= 10 3 µm 1 µm = 1,000 nm= 10 3 nm 1 mm = 1,000,000 nm = 10 6 nm 1 m = 1,000,000 µm = 10 6 µm 1 m = 1,000,000,000nm = 10 9 nm

9. 10 -3 m = ______ m = _____mm 10 -6 m = ______ m = _____ mm 10 -9 m = ______ m = _____ mm

10. 10 -3 m = 0.001m = 1 mm 10 -6 m = 0.000001 = 1 µm 10 -9 m = 0.000000001= 1nm

11. Electron microscope range: _____ to _____ Light microscope range: _____ to _____ Unaided human eye range : _____ to _____ Unaided?

12. Electron microscope range: 1 nm to 100 µm Light microscope range: 1 µm to 1 mm Unaided human eye range: 1 mm upwards

13. Name that micrograph Light microscope or Electron microscope?

14. 19

15. 19 Light microscope Unstained

16. 20

17. 20 Light microscope

18. 21

19. 22

20. 22 Light microscope

21. 23 Tick

22. 23 Electron microscope Tick

23. 24

24. 24 Electron microscope

25. 25

26. 25 Light microscope

27. 26

28. 26 Electron microscope

29. 27 Aphid

30. 27 Electron microscope Aphid

31. 28

32. 28 Light microscope

33. 29

34. 29 Light microscope

35. 30

36. 30 Electron microscope

37. 31

38. 31 Electron microscope

39. 32

40. 32 Electron microscope

41. 33

42. 33 Electron microscope

43. 35

44. 35 Light microscope

45. 36

46. 36 Light microscope

47. 37

48. 37 Light microscope

49. THE END!

50. Magnification Magnification = ocular lens x objective lens A microscope has an eyepiece lens with a power of 20X.  The objective lens being used has a power of 10X. What is the total magnification?

51. Using Scale Bars Using a scale bar: The image at right is of a virus-like particle. The bar represents 50nm . It is observed under x200. Calculate the actual size of the image. 50nm

52. Image is 50nm in diameter Using a scale bar: 50nm Using Scale Bars

53. A specimen is 1cm in diameter under a power of x600. What is the actual size of the specimen?

54. Magnification: I AM Watch yo units!! Image size Actual size Magnification Measure with ruler Need to figure this out Given to us

55. Measure the size of the image. …..1cm (10mm ) 2. Find the actual size of the sample I/AM I=10mm M=600 A=? A=10mm/600 = 0.0167mm =16.67 µm

56. Your Turn!