Mass spectroscopy

This chapter contains sections titled:

Introduction

Fundamentals

Ionization Techniques

Electron Impact and Chemical Ionization

Atmospheric Pressure Ionization

Electrospray

Atmospheric Pressure Chemical Ionization

Photoionization

Multiple Ionization Source

Desorption Electrospray and Direct Analysis in Real Time

Matrix Assisted Laser Desorption Ionization

Mass Analyzers

Quadrupole Analyzers

Triple Quadrupole Mass Analyzer

Ion Trap Mass Spectrometry

Triple Quadrupole Linear Ion Trap

Time of Flight Mass Spectrometry

Fourier Transform Mass Spectrometry

Fourier Transform–Ion Cyclotron Resonance Mass Spectrometry

Orbitrap Mass Spectrometer

Ion Detectors

Practical Aspects and Applications in Bioanalysis

Introduction

Quantitative Analysis in Biological Matrices

Drug Metabolism

Analysis of Proteins

Perspectives

Common Definitions and Abbreviations

1. BIOANALYTICAL TECHNIQUES ASSIGNMENT ASSIGNMENT TOWARDS PARTIAL FULFILMENT OF 2 nd Internal Examination ASSIGNMENT REPORT FOR SEMESTER –IV M.Sc. ZOOLOGY(2 ND year) NAME-AKANKSHA ROUT REG. NO.-190705180139 SECTION – B CAMPUS –BBSR GUIDED BY- M r . Bibhudutta Mishra

2. mass spectrometry in bioanalysis Akanksha Rout Centurion University Of Technology and Management , BBSR Core Course-Bioanalytical techniques M.Sc Zoology (4 th semester) 2019-2021 Sec B Registration no:190705180139

3. contents

4. ADVANTAGES DISADVANTAGES Molecular weight & formula analysis Sample destruction Qualitative and Quantitative analysis Sample always desired to be in the gaseous form. Less amount of sample is taken. Complex and expensive or high cost not cost effective Less than 1 minute for analysis. Should maintain vacuum throughout the process.

5. INstrumentation

6. INstrumentation

7. conclusion Mass spectrometry is thus, used for the study of charged molecules and fragment ions. Thus, it is useful for the identification of an unknown compound.

8. applications

9. conclusion Mass spectrometry is thus, used for the study of charged molecules and fragment ions. Thus, it is useful for the identification of an unknown compound . Thus, showing its high applicability and usage in the field of : Trace Gas Analysis Pharmaceutical Industry Space exploration Forensic Toxicology Archaeological Dating

10. ACKNOWLEDGEMENT ACKNOWLEDGEMENT I would like to express my special thanks of gratitude to my Dean Dr. Yashaswi Nayak as well as the HoD Dr. Sitaram Swain (Head of the Department) as well as our subject teacher Mr. Bibhudutta Mishra who gave me the golden opportunity to do this wonderful project on the topic Mass Spectroscopy, which also helped me in doing a lot of research and it helped to know about so many new things. I am really thankful to them. Secondly I would also like a thank my Department friends who helped me lot in finishing this project within the limited time and resources.It helped me increase my knowledge and skills. *THANKS AGAIN TO ALL WHO SUPPORTED*

11. THANKYOU