MCQs on DNA Fingerprinting.pdf

DNA fingerprinting is a technique used to identify and analyze the variations in various individuals at the level of DNA. DNA fingerprinting involves identifying differences in some specific regions in DNA sequence called as repetitive DNA because, in these sequences, a small stretch of DNA is repeated many times.

1. Multiple Choice Questions & Answers (MCQs) focuses on
“Molecular Basis of Inheritance – DNA Fingerprinting”.
Author: Dr. Rajendra Chavhan, Department of Zoology, RMG College, Nagbhid
1. What are the differences in the specific regions of DNA sequence called during DNA
finger printing?
a) Non repetitive DNA
b) Repetitive DNA
c) Satellite DNA
d) Histone DNA

Answer:b
Explanation: In DNA finger printing, the differences in the specific regions of the DNA
sequence are called as Repetitive DNA. In this part, a small structure of the DNA is repeated
many times. These repetitive structures can be separated from the bulk genomic DNA. This is
done by observing the different peaks achieved by them during the density gradient
centrifugation.
2. What are the small peaks achieved by the repetitive DNA during the density gradient
centrifugation process of DNA finger printing known as?
a) Non repetitive DNA
b) Trough
c) Satellite DNA
d) Histone DNA

Answer:c
Explanation: The genomic DNA forms a high peak in the density gradient centrifugation.
Whereas, the various small peaks which are formed are collectively called as the satellite DNA.
3. The sequences of satellite DNA do not code for proteins.
a) True
b) False

Answer:a
Explanation: The small peaks formed by the DNA when kept in the density gradient
centrifugation are called as satellite DNA. These DNA sequences do not code for proteins. On
the contrary, they form a large portion in the genome of humans.
4. How DNA can be as a useful tool in the forensic applications?
a) Showing the same degree of polymorphism with hair follicles
b) Showing different degrees of polymorphism with saliva
c) By not possessing any hereditable information
d) By the presence of lysozymes in it

Answer:a
Explanation: The double helical structure of the DNA present on saliva, hair follicles, bones,
blood and sperm serve as a useful took in the forensic studies. This can be done as the DNA
from an individual’s tissue shows the same degree of polymorphism. These polymorphic
characters are inheritable from parents to their children.
5. How does polymorphism arise?
a) Mutations
b) Recombination
c) Diploidy

2. d) Haploidy

Answer:a
Explanation: Polymorphism arises due to mutations. It can also be known as the discontinuous
variation that is seen among the individuals of a species.
6. Which tissue samples are used for DNA fingerprinting?
1. Hair
2. Skin
3. Blood
4. All of the above
The person's fingerprints are necessary in the final step of DNA fingerprinting.
1. True
2. False
DNA fingerprinting can be used to solve paternity-related disputes.
1. True
2. False
DNA fingerprinting cannot identify a person after his death.
1. True
2. False
What is PCR?
1. Plain chain Reaction
2. Ploymerase chain reaction
3. Police custody remarks
4. Paternal choice reaction
RFLP in DNA fingerprinting refers to-
1. Restriction fluorescent length polymorphism
2. Restriction fragment length polymorphism
3. Restriction fluorescent laser polymorphism
4. None of the above
VNTR refers to Variable Number of Tandem Repeats.
1. True
2. False
SNP can be used to determine a person's susceptibility to certain diseases.
1. True

3. 2. False
DNA fingerprinting can be done on body secretions.
1. True
2. False

1. DNA fingerprinting recognizes the differences in
(a) satellite DNA
(b) bulk DNA
(c) Repetitive DNA
(d) both (a) and (c)
Answer: (d)
2. This force can stabilize a DNA double-helix
(a) Hydrophilic sugar-phosphate groups are found on the exterior of the helix where interaction
with water occurs
(b) Hydrophobic bases are present in the interior of the helix, each base-pair is stabilized by the
same number of hydrogen bonds
(c) covalent base stacking interactions may take place between neighbouring bases within the
same strand in the helix
(d) non-covalent N-glycosidic bonds may form between nitrogenous bases in opposite strands in
the helix
Answer: (a)
3. In this type of DNA replication, of the two newly formed molecules, one is purely a new
one and the other is an old one
(a) dispersive
(b) conservative
(c) semiconservative
(d) both (b) and (c)
Answer: (c)
4. The process of DNA replication is affected by an enzyme known as
(a) Mutase
(b) Ligase
(c) Polymerase I
(d) Ribonuclease
Answer: (c)
5. A DNA molecule in which both strands have radioactive thymidine is permitted to
replicate in an environment that contains non-radioactive thymidine. What is the right
number of DNA molecules which possess some radioactive thymidine post three
duplications?
(a) one such molecule
(b) two such molecules
(c) four such molecules
(d) eight such molecules
Answer: (b)
6. If the DNA strand has nitrogenous base sequence ATTGCC, the mRNA will have?
(a) ATTGCA
(b) UGGACC
(c) UAACGG
(d) ATCGCC
Answer: (c)
7. The type of coiling in DNA is
(a) Zig-zag
(b) Left-handed
(c) Opposite
(d) Right-handed

4. Answer: (d)
8. In DNA, the enzyme which breaks the H2 bonds is
(a) Ligase
(b) Helicase
(c) Topoisomerase
(d) Polymerase
Answer: (b)
9. The total DNA comprises what amount of cytoplasmic DNA in cells?
(a) 95-99%
(b) 65-75%
(c) 45-50%
(d) 1-5%
Answer: (d)
10. The bases are held together in a DNA double helix by hydrogen bonds. These bonds
are
(a) Ionic bonds
(b) Covalent bonds
(c) Non-covalent bonds
(d) Van der Waals forces
Answer: (c)