MEGA (Molecular Evolutionary Genetics Analysis)

Mega 6, Multiple Sequences Alignment , Conserved Residues , Evolutionary Tree Construction , Unrooted Tree,Cluster Grouping,Inferring phylogenetic trees, Inferring ancestral sequences , Circular Tree ,

1. Athar Hussain
Roll No.2015
BS(Hon)
Bioinformatics and Biotechnology
Govt.College University Faisalabad
Email: [email protected]

2. Construction of Evolutionary tree Using MEGA

3. MEGA,MolecularEvolutionaryGeneticsAnalysis, is a
freely available software for conducting statistical analysis of
molecular evolution and for constructing phylogenetic trees.
MEGA is an integrated tool for conducting :
•Sequence alignment,
•Inferring phylogenetic trees,
•Estimating divergence times,
Introduction

4. Conti……..
•Estimating rates of molecular evolution,
•Inferring ancestral sequences,
•Testing evolutionary hypotheses.
MEGA is used by biologists in a large number of laboratories for
reconstructing the evolutionary histories of species and inferring the extent and
nature of the selective forces shaping the evolution of genes and species

6. Creation Of New Alignment

7. # Select Types of Sequence to be Align

8. Sequence Retrieving

10. Sequence Alignment Using ClustalW

11. ClustalW Parameters Setting

13. Multiple Sequence Alignment

14. Export Alignment to MEGA Format

15. Save MEGA format File

16. Construction Of Phylogenetic Tree Using
Maximum Likelihood Method

17. Opening MEGA format File which we Saved

18. Parameter Setting For Phylogenetic Tree

19. Progressing Tree

20. Rectangular Phylogenetic Tree
Showing the Conserved
Residues

21. Circular Tree Construction
From Rectangular Tree

22. Final Circular Phylogenetic
Tree After Grouping Genes
Different color represents
different Groups