Micro propagation

Micro propagation in hi-tech horticulture

1. Presentation on Stages and steps involved in Micro Propagation COURSE NAME : HI-TECH HORTICULTURE COURSE CODE: EC-35 SUBMITTED BY: Sweta Pattanaik

2. INDEX Introduction Stages in Micropropagation Steps in Microprogation Advantages Disadvantages

3. INTRODUCTION: ‘Micro-propagation has many synonyms - tissues culture, mass propagation, in-vitro culture, cloning. In vitro clonal propagation. It is practices of rapidly multiplying stock plant material to produce a large number of progeny plants, using modern plant tissue culture methods. Widely used for orchids, ferns, many interior foliage plants. Commercialization of Micro-propagation 1970s-1980s Murashige ( 1974 ) Broad commercial application

4. Stages of Micropropagation: It is complicated process and mainly involves 3 stages ( I, II and III ). Some others added two more stages (stage and IV ). Stage I ( Establishment of Explants ) Stage 0 ( Selection of explants ) Stage II ( Shoot multiplication ) Stage III ( Rooting ) Stage IV ( Acclimatization )

5. Diagrammatic representation of stages of micropropagation:

6. Steps of Micropropagation : In short, steps of micropropagation can be divided into 4 distinct stages. Selection of suitable mother plant Proliferation on medium Transfer of shoot to a rooting medium Transfer of plant to soil By adventitious root Through callus culture By apical and auxilary bud

7. Contd.

8. ADVANTAGES : Production of many plants that are clones of each other. Produce disease free plant. Increase yield of plants. Plant material without any danger of sickness. Genetically identical plants. Agronomical advantages.

9. DISADVANTAGES : Labour operating cost. Protocol not optimize for all species. Expensive to setup. Facilities required. Infected plants sample can produce infected progeny. Grow small amount of tissue at high cost.