Mycoplasma

Presentation

1. “Mycoplasma”


Seminar presented by

Ashish Diwakar
M.Sc.Microbiology II Semester


School of Studies in Microbiology
Vagdevi Bhavan,
Vikram University,Ujjain (M.P.)

2. Synopsis
• Introduction of Mycoplasma
• History of Mycoplasma
• Morphology of Mycoplasma
• Chemical composition of mycoplasma
• Classification of Mycoplasma
• Reproduction of Mcoplasma
• Structure of Mycoplasma
• Pathogenecity of mycoplasma
• Treatment
• Conclusion
• References

3. Introduction
• Unicellular Prokaryote
• Gram-Positive Bacteria
• Lack cell wall- Pleomorphic
• Osmotic Shock / Detergent-Sensitive
• Antibiotics-Tetracyclin / Chloremphenicol-Sensitive
• They are the smallest bacteria capable of autonomous growth

• Facultative anaerobes / obligate anaerobes
• Low G+C%, 23-41%
• Mostly require sterol for their growth
• (but some genera do not require sterol – such as
Acholeplasma, Asteroleplasma, Mesoplasma)

4. History
• Discovered by Pasture in 1843.
• Isolated from pleural fluid of cattle.
• It was called as Mycoplasma mycoides.
• Nocard & Roux first cultured Mycoplasma in1898.
• In 1929, Nowak called them PPLO.

5. Morphology
Size :- 300nm to 0.2nm
Shape :- Coccoidal,Diploform,Filamentus,Spiral
shape &Teardrop shape.

6. Colonical appearance & Colony
Cheracterstics:-
• Colony developed on the solied Medium.
• Show nippled or fried egg colony.

7. Chemical Composition
• Protein - 40-60%
• Carbohydrate - 0.1%
• DNA Content - 3-7%
• Lipid - 8-20%

8. CLASSIFICATION
Kingdom :Bacteria
Division :Firmicutes
Class :Mollicutes
Order :Mycoplasmatales
Family :Mycoplasmataceae
Genus :Mycoplasma

9. Reproduction of Mycoplasma
Binary fission
Budding or Round body formation
Filamentous formation

10. BINARY FISSION

11. STRUCTURE OF MYCOPLASMA

12. Pathogenesity
• Extra cellular parasite
• It Causes infection in Respiratory tract & Urinogenital tract
• In Respiratory tract Cause Pneumonia
• In Urinogenital tract Cause Male sterility
Diseases Causeing Mechenism of
Mycoplasma
Attachment :-
Host cell - Have - Glycoprotein

13. Penetration:-
• Mucosa membrane penetrate
• Attach on epithelial cells
• Growth on epithelial cells
• And destroy epithelial cells
• Cause Pneumonia
Treatment:-
• Tetracyclin / Chloramphenicol / Erythromycin

14. Conclusion
• Mycoplasma are peculier type of bacteria
• as they requiring sterol for growth
• They are cell wall less
• some are pathogenic (humen diseses)
• some are cattle/plants/insects parasite also

15. References
Willey J.M.,L.M.Sherwood & C.J. Woolverton
(2008) Prescott, Harly & Klein’s
Microbiology,Seventh Edition,MC Graw Hill,
Page no.571
PELCZAR M.J.,E.C.S. CHAN & N.R. KRIEG
2007 Microbiology Fifth Edition,MC Graw
Hill,Page no.281,805
Madigan,M.T.,Martinko,J.M.and Parker,
(2000) Brock Biology of Microorganisms,Sixth
Edition Prentice Hall International Inc.