Ninhydrid test of protein

1. >> 0 >> 1 >> 2 >> 3 >> 4 >>

2. *To proof there is free amino acids in protein

3. *Protein + Ninhydrin + Heat Purple
complex of soul

4. WHAT IS
THE NINHYDRIN
TEST OF
PROTEIN

5. Page 5
Ninhydrin

Ninhydrin (2,2-Dihydroxyindane-1,3-dione)
is a chemical used to detect ammonia or
primary and secondary amines. When
reacting with these free amines, a deep blue
or purple color known as Ruhemann's
purple is evolved. Ninhydrin is most
commonly used to detect fingerprints, as
amines left over from peptides and proteins
(terminal amines or lysine residues)
sloughed off in fingerprints react with
ninhydrin.

6. Protein
•Proteins are complex, specialized
molecules composed of carbon,
hydrogen, oxygen, and nitrogen. Many
proteins also contain sulfur. The building
blocks of proteins are the amino acids.

7. *Rack tube
*A test tube
*Pipette drops
*Heater
*Tube clamps

*Albumin 2%
*Gelatin 2%
*Pepton 1%
*Ninhydrin reaction 0,1%

8. *3 provided a clean test tube and
dry, 2ml subtance test
* Added 5 drops of ninhydrin
reaction
*Then, burn on during ±5 minute
*Seen the colour results

9. No. Test Subtance
Result Ninhydrin
Test
Free
amimo
acid (+/-)
1. Albumin 2% Purple +
2. Gelatin 2% Purple +
3. Pepton 1% Purple +

10. >> 0 >> 1 >> 2 >> 3 >> 4 >>

11. CONCLUTION
From the results of an experiment in getting results :
•Albumin + ninhydrin solution + heat purple
•Gelatin + ninhydrin solution + heat purple
•Pepton + ninhydrin solution + heat purple
So the solution is a positive reaction is Albumin, Gelatin
and Pepton.

12. *

13. Leader Winna Rismala Yeniar
Moderator Riyafi Rosmiati
Member Annisa Nurul Chaerani
Dewita Novitasari


Thank’s to Mr. Ryan & all my friend




Design By

Annisa Nurul Chaerani






SEKOLAH TINGGI ILMU KESEHATAN
JENDERAL AHMAD YANI
2010