NIOSOME, ITS PREPARATION AND EVALUATION

This Slide is Prepared by Suman Jyoti Sarmah

1. NIOSOME, ITS PREPARATION AND
EVALUATION







SUBMITED BY
Name: Suman Jyoti Sarmah
Class: M.PHARM 2
nd
Semester
Roll No: 180520011012
Institute: GIPS Azara

2. DEFINITION & STRUCTURE OF NIOSOMES
GENERAL CHARACTERISTICS
ADVANTAGES & DISADVANTAGES
METHODS OF PREPARATION
FACTORS AFFECTING STABILITY OF NIOSOMES
EVALUATION OF NIOSOMES
REFERENCE
CONTENT

3. Niosomes are synthetic microscopic vesicles consisting of an
aqueous core enclosed in a bi layer consisting of cholesterol and
one or more nonionic surfactants.
Vesicles are prepared from self assembly of hydrated non ionic
surfactants molecules.
Niosomes may be unilamellar or multimellar.
DEFINITION

4. Novel drug delivery system, in which the drug is encapsulated in a
vesicle which is composed of a bi-layer of non-ionic surface active
agents.
These are very small, and microscopic in size.
Although structurally similar to liposome, they offer several
advantages over them.
Basic structural components are- Non ionic surfactant, cholesterol,
charge inducing molecule.
STRUCTURE OF NIOSOMES

5. Biocompatible, biodegradable & non-toxic.
Niosomes can be characterized by their size distribution studies.
High resistance to hydrolytic degradation.
The properties of niosomes depends both on composition of the
bilayer & on method of their preparation.
GENERAL CHARACTERISTICS

6. Targeted drug delivery can be achieved
Reduced dose is required to achieve the desired effect.
Subsequent decrease in the side effect.
Improve the oral bioavailability of poorly soluble drugs.
Enhance the skin permeability of drugs when applied topically.
The surfactants used and also the prepared niosomes are
biodegradable, biocompatible and non-immunogenic.
They are osmotically active and stable.
ADVANTAGES OF NIOSOMES

7. Time consuming.
Requires specialized equipment.
Inefficient drug loading.
DISADVANTAGES OF NIOSOMES

8. Ether Injection Method
Film Method/ Hand Shaking Method
Sonication
Heating Method
Multiple Membrane Extrusion Method


METHODS OF PREPARATION

9. Ether Injection Method
A solution of the surfactant is made by dissolving it in diethyl
ether.
This solution is then introduced using an injection into warm
water or aqueous media.
Vaporization of the ether leads to the formation of single layered
vesicles.

10. Film Method/ Hand Shaking Method
Mixture of surfactant and cholesterol, dissolved in organic
solvent in a round bottomed flask.
Organic solvent is removed by low pressure/vacuum at room
temperature.
The resultant dry surfactant film is hydrated by agitation.

11. Sonication
A liquot of drug solution in buffer
Added to the surfactant/cholesterol mixture in a 10ml glass vial
The mixture is probe sonicated at 60 c for 3mins using a sonicator
with a titanium probe to yield niosomes.

12. Heating Method
Nontoxic, one step method.
Mixtures of non ionic surfactants, cholesterol and charge
inducing agent are added to an aqueous medium eg. Buffer,
H2O.
The mixture is heated while stirring at low shear forces.
Niosomes are formed.

13. Multiple Membrane Extrusion Method
Good method for controlling Niosomes size.
Mixture of surfactant, cholesterol and diacetyl phosphate in
chloroform is made into thin film by evaporation.
The film is hydrated with aqueous drug solution
Resultant suspension is extruded through polycarbonate
membranes.

14. FACTORS AFFECTING STABILITY OF
NIOSOMES

Nature of surfactant
Structure of surfactant
Temperature of hydration
Nature of encapsulated drug

15. Vesicle Diameter
Drug Content
Entrapment Efficiency
In Vitro Drug Release
Stability Studies
EVALUATION OF NIOSOMES

16. Vesicle Diameter
Vesicle size can be measured by using optical microscope with a
calibrated eyepiece micrometer. The vesicle size of 100 niosomes is
measured individually for all batches & its mean value is calculated.

Drug Content
Niosomal suspension equivalent to 10mg taken in a volumetric flask
of 100ml & volume was made up by phosphate buffer pH 7.4, after
that 1ml of this mixture was diluted to 10ml by phosphate buffer 7.4
& the % drug content was calculated or observed at using UV
spectrophotometer.

17. Entrapment Efficiency
After preparing Niosomal dispersion, un-entrapped drug is
separated by- Dialysis or using Centrifugation.

In Vitro Drug Release
It can be determine by membrane diffusion technique. A dialysis
sac is washed and soaked in distilled water. The vesicle suspension
is pipetted into a bag and sealed. The bag containing the vesicles is
placed in 200ml of buffer solution with constant shaking at 25 c or
37 c. At various time intervals, the buffer is analyzed for the drug
content by an appropriate assay method.

18. Stability Studies
Optimized formulation preserved at refrigerated temperature &
room temperature for 30days. After 30days shape, % drug
remaining & % entrapment efficiency of vesicles were measured.
The results were compared with the initial shape, % drug
remaining & % entrapment efficiency of both samples.

19. Niosomes are novel drug delivery system having more
advantages than the other form of formulation of nano
particles. It can be use for the targeted drug delivery system,
also for the drugs having poor solubility so it is widely used.

CONCLUSION

20. 1) A. Ahmed, Optimization of piroxicam niosomes using central composite
design. International Journal of Pharmacy and Pharmaceutical Sciences, 2013;
5(3):229-236.
2) M. S., Formulation and in vitro evaluation of niosomes
containing oxcarbazepine. International Journal of Pharmacy and
Pharmaceutical Sciences, 2012; 4(3):563-567.

3) A. Attama, Formulation and Evaluation of Niosomes, Indian Journal of
Pharmaceutical Sciences, May 2011; 73(3):323-8

4) P. Sundaresan, evaluation of aceclofenac niosomes prepared by various
techniques. International Journal of Pharmaceutical Sciences Review and
Research, 2012; 16(1):75-78

REFERENCE

21. THANK YOU