Oxidative phosphorylation

1. BIOLOGICAL OXIDATION – 3 ▪ OXIDATIVE PHOSPHORYLATION ▪ INHIBITORS ▪ UNCOUPLERS RITTU CHANDEL 19-03-2013

2. OVERVIEW 2 CARBOHYDRATES FATS AMINO ACIDS CELLULAR RESPIRATION ENERGY OF OXIDATION DRIVES THE SYNTHESIS OF ATP

3. Biochemical anatomy of mitochondria 3 POWER HOUSE 0f cell

4. OXIDATIVE PHOSPHORYLATION Transport of electron through electron transport chain is linked with release of free energy Process of synthesizing ATP from ADP and Pi coupled with ETC Site : complex V of inner mitochondrial membrane 4

5. SUMMARY:ELECTRON TRANSPORT CHAIN 5

6. 6 ATP

7. TRANSFER OF ELECTRON FROM NADH THROUGH ETC 7

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9. PROTON MOTIVE FORCE 9

10. CHEMIOSMOTOIC THEORY 10

11. COUPLING OF ELECTRON TRANSFER AND ATP SYNTHESIS 11

12. FoF1 ATPase or ATP synthase or Complex V 12

13. ROTATORY OR ENGINE DRIVING MODEL 13

14. TOP VIEW OF F1 14

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16. BINDING CHANGE MECHANISM-PAUL BOYER 16

17. 17 ATP ADP TRANSLOCASE

18. MECHANISM OF ATP - ADP TRANSLOCASE 18

19. P/O RATIO Number of molecules of ATP synthesized per pair of electron carried through ETC P/O for oxidation of NADH = = 2.5 P/O for oxidation of FADH2 = 1.5 19

20. REGULATION OF OXIDATIVE PHOSPHORYLATION Availability of ADP regulates the process If ATP is low, ADP is high it proceeds a rapid rate 20 TI OXYGEN UPTAKE TIME rest ADP increased Returning to rest

21. UNCOUPLERS 21

22. UNCOUPLER 22

23. UNCOUPLERS PHYSIOLOGICAL Thyroxine in high doses Thermogenin or UCP 1 Unconjugated hyperbilirubinemia SYNTHETIC 2,4 – dinitrophenol 2,4 – dinitrocresol FCCP, Gramicidin A,high doses of aspirin 23

24. INHIBITORS Oligomycin,Dicyclohexylcarbodiimide aurovertin 4-chloro – 7- nitrobenzofurazan Atractyloside bongregate 24

25. summary 25

26. CHEMICAL COUPLING By E.Slater A high energy phosphorylated intermediate is common to both ETC and phosphorylation of ADP Why was this not accepted ? 26

27. SUBSTRATE LEVEL PHOSPHORYLATION In glycolysis In TCA cycle Conversion of succinyl CoA to succinic acid Lohman reaction Myokinase reaction 27

28. OXIDATIVE PHOSPHORYLATION DISEASES 28 EYE Ophtalmoplegia retinopathy LIVER KIDNEY glomerulopathy PANCREAS Diabetes mellitus INNER EAR Sensorineural loss BRAIN Seizures,ataxia,stroke SKELETAL MUSCLE Myopathies HEART Conduction disorders

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30. BIBLIOGRAPHY SATYANARYAN VASUDEVAN PANKAJA NAIK LIPPINCOTT BHAGVAN MARKS GRISHAM LEHNINGER STRYER VOET HARPER THANK YOU 30

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