PLATELET ACTIVATING FACTOR

PAF

1. ASSIGNMENT PRESENTED TO Dr . TARIQUE MAHMOOD ASSOCIATE PROFESSOR & HEAD Mrs. ARSHIYA SHAMIM ASST. PROFESSOR PRESENTED BY MULAYAM SINGH DOCTOR OF PHARMACY (2 nd YEAR ) SESSION : 2018-19 PHARMACOLOGY - 1 PRY - 206 INTEGRAL UNIVERSITY , LUCKNOW FACULTY OF PHARMACY M.singh PRESENTED BY MULAYAM SINGH SADIYA PERWEEN NAUREEN FATIMA DOCTOR OF PHARMACY (2 nd YEAR)

2. TOPIC M.singh Platelet activating factor: Mechanism of action & Clinical uses

3. INTRODUCTION Platelet-activating factor (PAF) is a lipid mediator that is well-known for its ability to cause platelet aggregation, inflammation, and allergic response at very low concentrations (approaching picomolar). M.singh

4. platelet Platelets  are tiny blood cells that help your body form clots to stop bleeding. If one of your blood vessels gets damaged, it sends out signals that are picked up by platelets . The  platelets  then rush to the site of damage and form a plug, or clot, to repair the damage. M.singh

5. FUNCTION OF platelet M.singh

6. MECHANISM OF ACTION M.singh In pharmacology, the term  mechanism of action  (MOA) refers to the specific biochemical interaction through which a drug substance produces its pharmacological effect. A mechanism of action  usually includes mention of the specific molecular targets to which the drug binds, such as an enzyme or receptor.

7. CLINICAL USE relating to or based on work done with real patients : of or relating to the medical treatment that is given to patients in hospitals, clinics, etc. : requiring treatment as a medical problem. : of or relating to a place where medical treatment is given : of or relating to a clinic. M.singh

8. What is platelet activating factor..?? Platelet-activating factor is a potent biological mediator that exerts its effects in a variety of cells and tissues. Platelet-activating factor, also known as PAF, PAF- acether or AGEPC (acetyl- glyceryl -ether- phosphorylcholine), is a potent phospholipid activator and mediator of many leukocyte functions, including platelet aggregation degranulation inflammation, and anaphylaxis. continue…. M.singh

9. M.singh continue…. It is also involved in changes to vascular permeability, the oxidative burst, chemotaxis of leukocytes, as well as augmentation of arachidonic acid metabolism in phagocytes. PAF is produced by a variety of cells, but especially those involved in host defense, such as platelets, endothelial cells, neutrophils, monocytes, and macrophages.

10. Synthesis of PAF PAF is synthesized from precursor phospholipids present in cell membrane by the following reactions: Membrane Acyl-glycero phosphocholine Phospholipase A2 Fatty acid Lyso PAF PAF- acetl transferase PAF Acetyl CoA CoA The second step is rate limiting. Antigen-antibody reaction and a variety of mediators stimulate PAF synthesis in a Ca2+ dependent manner on demand: there are no preformed stores of PAF. In contrast to eicosanoids , the types of cells which synthesize PAF is quite limited—mainly WBC, platelets, vascular endothelium and kidney cells. M.singh

11. PAF is degraded in the following manner : PAF Lyso PAF Acyl glycerophosphocholine (gets incorporated in the membrane) PAF acetyl- hydrolase Acyl - transferase Acetate Fatty acid Degradation of PAF M.singh PAF :- platelet activating factor Lyso PAF :- Lysophosphatidylcholine

12. M.singh What is PAF audience?

13. M.singh audience

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