practical1_Safety (1).pdf Rahaf tawfeek.

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FieldofMedicine
MedicineAndSurgeryProgram
Lecture:labsafetypractical1
Date:11/2/2024Dr:Moatazmaherand Abeer Eid

Introductiontopracticallabs
(Labsafety)
MedicalBiochemistry&MolecularBiologyDepartment

Objectives:
Bytheendofthislabsession,thestudentwillbeableto:
1.Overviewthebasicsofsafetyinthelaboratory
2.Identifygenerallabsafetypractices.

1.BasicRulesofLabSafety
WhatisLabSafety?
•Thelaboratorysettingpresentsseveralhazardsthatmustbeconsideredwhen
performinganylabexercise.
•Laboratorysafetyincludesproceduresthataredesignedfortheprotectionof
alllaboratorypersonnelandthesafeuseoflaboratoryequipment.
•Italsoinvolvesstrictprotocolsonthedisposalofcontaminatedmaterialsand
chemicals.

1.BasicRulesofLabSafety
×Safetyglassesmustbeworninthelaboratoryallthetimes
×ALabcoatshould bewornallthetime
×Protectyourfeetbywearing“closedtoed”shoes
×Tiebacklonghair
×Neverruninthelaboratory

1.BasicRulesofLabSafety
×Neverworkinthelabwhenthesupervisorisnotpresent.
×Eating,drinkingandsmokingarenotallowed
×Reportallaccidents
×Alwaysdisposechemicalwastesinthecorrectmanner

1.BasicRulesofLabSafety
×Incaseoffire:
1)Donotpanic
2)Ifthefiredistinguisherisrequiredleaveittothesupervisor
3)Ifyourclothescatchesfire,wrapyourselfwithfireblanket
(orcoat)androllontheground.
4)knowthelocationofsafetyequipmentinthelaboratory
classroom.

2.GeneralLabsafetyPractices
DON’T
×eat,drinkorsmokeinthelaboratory
×pipettebymouth
×leaveequipmentusingwater,gasorelectricityonovernight
×Nevertasteanythingortouchchemicalswithhand
×Neveraddwatertoconcentratedsulphuricacid.

2.GeneralLabsafetyPractices
Do(Always)
wearalabcoat&appropriateeyeprotection
Placebooksandpersonalitemsinthecabinet
Listentoyourinstructorcarefullyandstandinyour
properpositioninthelab.
Keepyourworkingareacleanandtidy.

2.GeneralLabsafetyPractices
Do(Always)
Weargloves
Usetheflamewithextreme
cautionandKeepyourheadand
clothingawayfromtheflame
Keephandsawayfromface,eyes
andclothesduringlab.
X √

2.GeneralLabsafetyPractices
Do(Always)
Readtheinstructionguide
aheadofperformingthe
experiment.
Useonlyclean&dryglassware
andneverusebrokenglassware.
Keepbrokenglassware&sharps
separatefromotherwaste&
disposethemofinthe
appropriatecontainers.
Openbottlesofchemicalsolutions
underwoodorwhereventilation
isavailable.
Handleconcentratedacids&
liquorammoniawithcare.
Securethetopsofreagentbottles
immediatelyafteruse.

2.GeneralLabsafetyPractices
Do(Always)
Labelchemicalsclearlyandpermanently.
xNeverusechemicalsfromunlabeled
containers
xDonotplacelabelsontopofoneanother.
Washhandsafterusinganysubstanceshazardoustohealth,andon
leavingthelaboratory.

Whatistheimportanceofhandwashing?
Before,during,andafterthelab….
WhatarethestepsforCorrectHandWashing
Technique?

**Emergencyprocedures
Incaseofeyeinjury
Flusheyesimmediatelywith
plentyofwaterforatleast15
minutes.
Incaseofaccidentasanacidoralkali
burns
Washtheaffectedareaimmediately
withplentyofrunningwater.

**HazardSymbols

BestWishes