Presentación seminario biologia molecular

Seminario de biología molecular sobre cáncer colon rectal

1. Ana MaríaMazoBossio
Juliana Mejía
Third semester of medicine
Pontifical Bolivarian University of
Medellín

2. •Colon cancer ranks third among
global tumours and second in
cancer-related mortality
•The liver is the most common site
for distant metastasis
•C6orf15 is a novel gene that has
been reported only in Sjogren’s
syndrome and systemic lupus
erythematosus patients
Introduction

3. Introduction
β-catenin activation induces EMT
progression by altering
intercellular junctions,leadingto
the invasiveness of CRC. The
nuclear aggregation of β-catenin
is a crucial step in initiating the
transcription of LEF1-regulated
genes.

4. General objective
The aim of this study was to explore the potential of C6orf15 as a
therapeutic target for colorectal cancer

5. Métodos
Aislamiento y cuantificación de ARN mediante PCR
cuantitativa de fluorescencia en tiempo real
La PCR cuantitativa en tiempo real consiste en detectar y cuantificar una secuencia Diana
específica o niveles de expresión genica en una muestra, en este caso se hizo con líneas
celulares de Cáncer colorectal y 48 muestras de tejido colorrectal

6. Métodos
Aislamiento de proteínas y western blot
El western blot es una técnica de laboratorio en donde buscamos detectar una proteína
específica en una muestra, en este caso se utilizaron nuevamente líneas celulares
infectadas de cancer colorrectal, buscando identificar la presencia de algunas proteínas en
las muestras

7. Métodos
Tincióninmunohistoquímica (IHC)
En la tincioninmunohistoquimicase usananticuerposcon el fin de saber sihay ciertos
antígenosen unamuestra, en este métodose prepararonseccionesconsecutivasde tejido
con cancer colorrectaly tambiénse hizola respectivarecuperacióndel antígenopara
determinarsiestabapresenteo no

8. Métodos
Immunofluorescencia
La inmunofluorescenciaayudaa detectardeterminadosantígenosen unacelulao en la
muestrade un tejido, en este casose usaronseccionesde tejidoscon metástasis
hepaticas y con focosprimariosde cancer de colon para despuésde determinara través
de la pruebaqueantígenosestabanpresentes

9. Resultados

10. Resultados

11. Resultados

12. Resultados

13. Discussion
Authors What they said Agree or
disagree
Fabio S, Holm SJ, Lotus M,
O’Brien KP, Mona S.
The function of C6orf15 has not been clearly
elucidated, and previous studies have noted that it is
expressed in the skin and tonsils, which may be
associated with susceptibility to follicular lymphoma.
Wada T, Matsuda Y, Muraoka
M, TomaT, TakeharaK,
Fujimoto M, YachieA
Moreover, its absence may be associated with
desquamation. C6orf15 is predicted to be involved in
extracellular matrix composition and collagen V-and
fibronectin binding activities.
Yang S, Liu Y, Li MY, Ng CS,
Yang SL, Wang S, Zou C,
Dong Y, Du J, Long X, Liu LZ,
Wan IYP, MokT, Underwood
MJ, Chen GG.
Despite multifaceted advances in the treatment of
CRC, it remains one of the most aggressive
malignancies and is characterized by rapid tumour
recurrence and early metastasis. Liver metastases are
the leading cause of death in colorectal cancer patients

14. Conclusions
•The identification of the C6orf15 protein as a hepatic
metastatic marker in colorectal cancer was achieved.
•Thanks to the identification of gene C6orf15 helps in the
identification of liver metastasis and the activation of
different processes of the same, helping us with a better
treatment.
•The study of genes is of utmost importance in many
medical studies and mainly in cancer, helping for a better
visualization of its origin, evolution and the way in which
it can affect different pathways and other parts of the
body.