Presentasi Federation of Islamic Medical Association 2015

PPT tentang BFGF

1. S
Proliferative Effect of Basic Fibroblast Growth Factor
(bFGF) Supplementation on Peripheral Blood-Derived
Mesenchymal Progenitor Cells (MPC)
Prima Eriawan Putra
Faculty of Medicine
YARSI University
Prima Eriawan Putra • Reynaldi • Qeis Ramadhan • Vilona Afrita Zilmi •
Milatianingrum • Indra Kusuma • Restu Syamsul Hadi • Yurika Sandra

2. Intro
SIslam is an integral part in medical practice and research.
History proves their correlations.
SBasic science needs more recognition from muslim
scientists.

3. Background
SPeripheral blood aspiration is less invasive than bone
marrow aspiration to obtain stem cells (Maurice, et al., 2007)
SMPC cells are capable to differentiate into mesenchymal
lineage cells (osteoblast, chondroblast, adipocyte) (Battler
and Leor, 2006)
SbFGF promotes growth of various cells, notably bone
marrow stromal cells (Yuan, et al., 2013)

4. Objective
SThe aim of this study was to observe bFGF effect on
MPC cell proliferation

5. Materials and Methods
SStudy design: experimental
STechnical: MPC cells were divided into 6 groups








SAbsorbance number was measured using microplate
reader, and analyzed using Microsoft Excel 2011
MPC groups supplemented with 4
different bFGF concentrations
Unsupplemented group
(positive control)
H
2
O
2
treated group
(negative control)

6. Materials and Methods
http://www.asnahlearning.com/images/
Phlebotomy01.jpg
Blood aspiration
http://www.imagesource.com/Doc/IS0/Media/
TR6/2/4/5/c/IS09AJ1U7.jpg
Cell culture in DMEM +FBS
4 different concentrations of
recombinant bFGF
Absorbance reading with a Microplate
Reader

7. Results
1 2
Fig 1 and 2. Microscopic imaging of peripheral blood-derived mesenchymal
progenitor cells

8. Results
WST-1 Absorbance Value
0
0,55
1,1
1,65
2,2
Day In Vitro
DIV 2 DIV 4 DIV 7
Fig 3. Effect of bFGF on MPC-PB cell proliferation
8 ng/mL
16 ng/mL
32 ng/mL
2 ng/mL
Control (+)
Control (-)

9. Results
Fig 4. MPC-PB cell proliferation after 7 day in vitro. Statistical
significance *p<0,05 compared to positive control group
WST-1 Absorbance Value
0
0,75
1,5
2,25
3
bFGF Concentrations (ng/mL)
2 8 16 32 Control (+)Control (-)
*
*
* *

10. Conclusion
SbFGF can be used as a cell culture supplement for
peripheral blood-derived mesenchymal progenitor cell
SFurther study needed to investigate a panel of growth
factor or small molecules to replace serum as stem cell
culture supplement.