Presentasi Konferensi Layer 2024 - Rev. 1.1.pptx

About Avian Metapneumo Virus that infected QL Agrofood and how to deal with it

1. AMPV (Avian Metapneumo Virus) DATE : 5-6 MAY 2024 QL AGROFOOD INDONESIA DIVISION COMERCIAL LAYER FARM

2. HISTORY INITIAL DIAGNOSIS Swollen eyes, high mortality, and a drop in production. These are the clinical signs we noticed when we first made our diagnosis– Infectious Coryza. After a course of antibiotic treatment, mortality rate dropped. However, after 2 months , the signs reoccurred. And even worse, this time it had spread to pullet. In pullet, the severity of the clinical signs were much worse, with signs such as turbid discharge from nasal cavity, swollen eyes coupled with severe Colibacillosis infection . We have came to a conclusion, this case, it is not as simple as Infectious Coryza. It is- AMPV infection.

3. AMPV AMPV (Avian Metapneumovirus, also known as Swollen Head Syndrome) is a viral disease that causes catarrhal inflammation. The site of inflammation would be at the sinuses (infra and supra orbital). The first stage of inflammation causes oedema, hence the swollen face. This causes extreme discomfort to the bird and even worse, the inflamed site introduces bacteria to invade, leading to secondary bacterial infection.

4. Clinical signs and Pathologic Lesions Swollen eyes and face (asymetrical and symetrical when it cronic) . Clear exudate (Odorless) from nasal cavity Snicking and Sneezing Dull and thin Reduced feed intake Post mortem lesions: Chronic colibacillosis lesions – Airsacculitis , pericarditis, perihepatitis, peritonitis, salphingitis )

5. Clinical Signs Swollen face

6. Clear exudate from nasal cavity (odorless)

7. Pathological lesions Airsaculitis , pericarditis, perihepatitis

8. Peritonitis

9. Salphingitis in pullet

10. Salphingitis in layer

11. Impact of Ampv infection AMPV in normal situation Normally , AMPV infection causes damage to the respiratory tract, introducing secondary bacterial infection to the site. With the treatment of antibiotics, this issue can be resolved quickly. More so, the infected birds don’t even need AMPV vaccination because the antibody produced will be sufficient to fight off the disease. AMPV in QL Agrofood Layer But , in Agrofood Layer Farm this is not the case. AMPV infection in this farm is complicated with infection of virulent E.coli (APEC), which treatment of antibiotics can be futile most of the time.

12. Impact of Ampv infection PULLET High depletion ( Cum Dep 20% ) Reduced feed intake Suboptimal body weight dan uniformity LAYER High depletion Delayed in production (young flock) Low Production (max 75% HD)

13. LAB REPORT SEROLOGI PULLET

14. LAB REPORT SEROLOGI LAYER

15. Lab . Result of E .Coli Apec

16. SUMMARY HASIL REPORT LAB E. COLI APEC

17. ACTION TAKEN 1 Addition of AMPV vaccines in vaccination program: A MPV live vaccine on Day 5 A MPV killed vaccine on week 12 th Antibiotic treatment Details of the vaccines used: Avisan SHS ( Hypra ) : Live vaccine, turkey rhinotracheitis and swollen head syndrome Avisan TRT ( Hypra ) : Inactivated vaccine, Subtype B, Chicken origin, against avian pneumovirus

18. Weekly D epletion Age Weekly Deple tion (%) 1 week 0.3 2 weeks 0.14 3 weeks 0.15 4 weeks 0.12 5 weeks 0.07 6 weeks 0.16 7 weeks 0.15 8 weeks 0.96 9 weeks 4.21 10 weeks 2.36 11 weeks 2.44 12 weeks 1.09 13 weeks 0.82 14 weeks 1.12 15 weeks 0.42 16 weeks 0.21 17 weeks 0.24

19. Weekly P roduction Age Pro duction (%) 18 weeks 0.28 19 weeks 2.89 20 weeks 11.99 21 weeks 27.98 22 weeks 47.29 23 weeks 58.51 24 weeks 67.11 25 weeks 70.67 26 weeks 73.74 27 weeks 73.97 28 weeks 73.23 29 weeks 73.98 30 weeks 75.44 31 weeks 76.33 32 weeks 77.74 33 weeks 78.75 34 weeks 81.9 35 weeks 82.3 36 weeks 81.32 37 weeks 81.35 38 weeks 82.76 39 weeks 84.44 40 weeks 81.4

20. ACTION TAKEN 2 Another addition of aMPV vaccines. Usage of probiotic A MPV live vaccine on Day 5 and Week 7 th A MPV killed vaccine on Day 5 and Week 9 th Antibiotic treatment Probiotic treatment(customized probiotic profile) Probiotic contains Bacillus strain 747 with 81-100% inhibition ( Corellink ) to pathogenic E.coli isolated from farm.

21. Weekly Depletion Age Weekly Deple tion (%) 1 week 0.21 2 weeks 0.24 3 weeks 0.2 4 week 0.07 5 weeks 0.2 6 weeks 0.16 7 weeks 0.11 8 weeks 0.12 9 weeks 0.11 10 weeks 0.4 11 weeks 0.42 12 weeks 0.3 13 weeks 0.38 14 weeks 0.37 15 weeks 0.33 16 weeks 0.27 17 weeks 0.27

22. Weekly P roduction Age Weekly Produ ction (%) 18 weeks 0.22 19 weeks 1.15 20 weeks 18.04 21 weeks 44.51 22 weeks 67.94 23 weeks 82.05 24 weeks 83.68 25 weeks 85.63 26 weeks 89.33 27 weeks 91.92 28 weeks 91.39 29 weeks 92.21 30 weeks 91.84 31 weeks 91.89

23. CONCLUSION - Depletion Graph of Case, Action Taken 1, dan Action Taken 2 Case (Before action): AMPV outbreak happened at week 13 th , with persistent high depletion which does not reduce immediately. Action 1: AMPV outbreak happened earlier, which is on week 8 th , with high depletion as well. But this time, the depletion rate reduced fair quickly. Action 2: Normal depletion AMPV infection causes immunosuppression, leading to secondary bacterial infection especially pathogenic E.coli infection. AMPV diseases can be overcome with vaccination program, treatment of antibiotic and the correct probiotic.

24. TERIMA KASIH