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About This Presentation

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Slide Content

Microbiology Quiz - Bacteriology

Define ASYMPTOMATIC STAGE
•Infection with negligible inflammation.
•there are no symptoms.

Name the water soluble pigment
in Pseudomonas aeruginosa
Pyorubin

Ureaplasma is
Causative agent of ?
Urethritis

When Enterococcus facalis grows in
media containing Esculin, it
hydrolyse the esculin &produce black
Colonies. Name the test?
Bile Esculin test

Define Febrille Sickness
Patient with an elevated body temperature
or fever.
Mental clounding of consciousness
A febrile seizure is
 a convulsion in a child
that's caused by a fever.
 

Find the Organisms which
causes Gas gangrene
Clostridium perfringens

Abbreviation GTTA
Monsours Gelatin Taurocholate
Trypticase Tellurite Agar

Causative agent of Shigellosis

What is Heat stable toxin ?
Toxins which can not be detroyed by heat

RIA,
ELISA,
HEMAGGLUTINATION
Mention the serological test
for N. gonorrhaeae ?

What is the result of Quellung
reaction in S. pneumonia ?
Capsule will swell and become more visible

Define Virulence Factor
Ability of an Organism to infect the
host and cause a disease

What is the uses of Lepromin test ?

What is Medusa Head Appearance
Edge of the
colonies appear
as tangled mass
of hairy curls

What is the common name for
Klebsiella pneumoniae
Friedlander’s bacillus

What is the Growth factor
required by H. influenza to grow

Name the Chlamydia infections
in man ?
1.Oscular infection
2.Respiratory infection
3.Genital infection

What type of bacterial morphology
and gram stain reaction is shown in
the picture below?

Gram Negative Diplococcus

Which of the following can be
identified by Milk ring test ?
1.Plague
2.Whooping tuberculosis
3.Brucellosis
Brucellosis

Borrelia burgdorferi is the
_____(shape) bacteria that is the
major cause of Lyme disease in
the United States

Borrelia burgdorferi is the spiral
shaped bacteria that is the major
cause of Lyme disease in the
United States

What is the name of infection
caused by Actinomyces
organism ?
NODNGSOEUE
Ans:
ENDOGENOUS

Find the type of disease in
Leprosy?
L__P__OM__T__US
__UB__RC__L__ID
LEPROMATOUS
TUBERCULOID

Who is the first person to
study about C. diphtheriae
KBESLWDINE
EDWIN KLEBS

Pseudomonas

MYCOBACTERIUM TUBERCULOSIS

CHLAMYDIA

BRUCELLA

Leptospira

Hemolytic uremic syndrome

Most common cause of Acute
osteomyelitis is
•Staphylococcus
•Neisseria
•Salmonella
•Meningococcus

Most common cause of Acute
osteomyelitis is
•Staphylococcus aureus
•Streptococcus
•Salmonella
•Pseudomonas

In
 O157:H7, what does 'O'
indicate?
•Flagellar protein
•Cell wall lipopolysaccharide
•Peptidoglycan matrix
•Capsular polysaccharide

In
 O157:H7, what does 'O'
indicate?
•Flagellar protein
•Cell wall lipopolysaccharide
•Peptidoglycan matrix
•Capsular polysaccharide

Which bacterial capsule is madeup
of amino acids ?
•Bacillus anthracis
•Pneumococcus
•H.infuluenza
•Neisseria

Which bacterial capsule is madeup
of amino acids ?
•Bacillus anthracis
•Pneumococcus
•H.infuluenza
•Neisseria

What is this test called and why?.
Also have connection with another
organism. what is it
Given that the horizontal
streak is Staphylococcus
aureus, what organism is
likely to be represented by
the “arrow” pointing towards
it?

CAMP test, after the people who were
involved in developing it (Christie,
Atkins & Munch-Peterson.)
It is likely to be Streptococcus
agalactiae (Group B
streptococcus). There is
synergistic
 haemolysis betwee
n the
 Streptococcus agalactiae
and the Staphylococcus aureus
producing an arrowhead
effect. The bottom left hand
streak is likely to be a negative
control such as Streptococcus
pyogenes.

The microorganism circled in the
image below is the causative agent of
syphilis. What is the scientific name for
this microbe?

Treponema pallidum

Splenectomised patients more
prone to infection with
–Gonococcus
–Meningococcus
–Staphylococcus
–Mexicoccus

Splenectomised patients more
prone to infection with
Splenectomised 
and hyposplenic 
patients 
are at
increased risk of life-threatening infection due to
encapsulated micro-organisms such as
Streptococcus pneumoniae (90%), Neisseria
meningitidis, and Haemophilus influenzae as well as
certain parasitic infections such as Malaria and
Babesiosis.

Have a look at this stain on a sputum
sample and answer the questions
below:
i) What type of stain is
demonstrated?
ii) What is the possible
diagnosis?

Ziehl-Neelson stain. Originally
invented by
  Franz Ziehl and then
Friedrich Neelson- Mycobacterium
tuberculosis

5(1).What is listeria?
•A strain of virus
•A strain of bacteria
•A strain of algae
•A strain of fungus

A strain of bacteria
Like the famous mouthwash Listerine,
 
Listeria
monocytogenes was named after antiseptic
pioneer Joseph Lister. It's a very common
bacterium with an unusual trait: It can grow at
refrigerator temperatures. And it can build up
in food-processing plants, where it can survive
for years

5(2). Character of a bacterial capsule is
•All bacteria have a capsule
•It is always composed of peptidoglycan
•It is an important protective mechanism
•It is responsible for gram stain reaction

Character of a bacterial capsule is
•All bacteria have a capsule
•It is always composed of peptidoglycan
•It is an important protective mechanism
•It is responsible for gram stain reaction

5(3). Gas Gangrene is caused by
–Clostridium perfringens
–Clostridium tetani
– Clostridioides difficile
–Clostridium tertium 

Gas Gangrene is caused by
Clostridium perfringens

5(4). Buruli ulcer is caued by
 
–Mycobacterium ulcerans
–Peptostreptococcus
–Helicobacter pylori
–S. aureus &
peptostreptococcus

Buruli ulcer is caued by
 
–Mycobacterium ulcerans
–Peptostreptococcus
–Helicobacter pylori
–S. aureus & peptostreptococcus

5(5). Traveler's diarrhea is caused by
•Enterotoxigenic E. coli (ETEC)
•Enteroaggregative E. coli (EAEC)
•Enteropathogenic E. coli (EPEC)
•Enterohemorrhagic E. coli (EHEC)
•Enteroinvasive E. coli (EIEC

Traveler's diarrhea is caused by
•Enterotoxigenic E. coli (ETEC)

5(6). Organism capable of
causing
  hemolytic uremic
syndrome (HUS)
•Enterotoxigenic E. coli O157:H7
•Enteroaggregative E. coli O157:H7
•Enteropathogenic E. coli O157:H7
•Enterohemorrhagic E. coli O157:H7
•Enteroinvasive E. coli O157:H7

Organism capable of
causing
  hemolytic
uremic syndrome
(HUS)
Enterohemorrhagic
E. coli O157:H7

5(7). Sereny test is done for
–Enterotoxigenic E. coli (ETEC)
– Enteroaggregative E. coli (EAEC)
–Enteropathogenic E. coli (EPEC)
–Enterohemorrhagic E. coli (EHEC)
–Enteroinvasive E. coli (EIEC)

Enteroinvasive E.
coli (EIEC)

5(8). Which bacterial capsule is
madeup of amino acids ?
•Bacillus anthracis
•Pneumococcus
•H.infuluenza
•Neisseria

Which bacterial capsule is made
up of amino acids ?
•Bacillus anthracis
It usually consists of
 polysaccharides but can be
composed of other materials such as
glycoprotein,
 polypeptide D-glutamic
acid
 in 
B. anthracis, and peptidoglycan and
muramic acid found in
 
E.coli 
bacterial capsule.

5(9). Food poisoning associated
with chinese restaurant fried rice
•Bacillus cereus
•Salmonella
•Staphylococcus aureus
•Vibrio parahemolyticus

Food poisoning associated with
chinese restaurant fried rice- clues

Food poisoning associated with
chinese restaurant fried rice
•Bacillus cereus
•Salmonella
•Staphylococcus aureus
•Vibrio parahemolyticus

5(10). An Injecting Drug User presents
to the
 ED with the lesion (as shown)
on his hand and arm. He also has a
fever.
What disease is very
important to exclude as a
cause of his lesion?

Cutaneous anthrax
•When
 
anthrax 
spores get
into the
 
skin, usually
through a cut or scrape, a
person can
develop
 
cutaneous anthrax.
This can happen when a
person handles infected
animals or contaminated
animal products like wool,
hides, or hair.
 
Cutaneous
anthrax 
is most common on
the head, neck, forearms,
and hands.
Bacillus anthracis

Mr.Rv has respiratory
infections for past 2 weeks.
What treatment doctor has
given to him?


Azithromycin

Mr. Vijay has conjunctivitis
sinusitis otitis for past 3 weeks.
What treatment doctor has
given to him?

Amoxyllin and haemophillus

Mr.X is 65 years old. He have
symptoms of fever,chills,swelling,
pain and drainage or pus formed In
a surgical site . Which organism can
cause these conditions?

Ans :Klebsiella pneumoniae

Mr. Dhilip is 35 years old. He
have a symptoms of bloody
diarrhea,fever and stomach pain.
Which organism can cause these
conditions?

Shigella dysenteriae

Mrs. Nandhini is 35 years old.
She has the symptoms of
profuse painful watery diarrhea
along with copious effort .
Mention the organism

Vibrio cholerae

Mr.Siddhu has
fever,chills,fatigue and
haemodynamic shock.What is
haemodynamic shock? and
mention the reason

Haemodynamic shock- low blood
pressure
Reason-bacterimia

Mr.Aadhi has continous or
intermittent fever with profuse
sweating,weakness,joint pain and
weight loss . The disease was
caused by brucellosis abortus.
Mention any 3 diagnostic tests used
for the lab diagnosis

RIA
ELISA
CFT

Mr.X has complaints of
malaise,Pete Hill rashes on skin
and mucosa,lesions in lungs,
joints and acute adrenal
insufficieny.What is the another
name of acute adrenal
insufficiency?

Waterhouse friderichsen
syndrome

The patients get rashes which
appears in chest and abdomen
on the 2
nd
and 3
rd
week of
infection. It is caused by
salmonella.What are the rashes
called?

Rose spots

A 78 year old man confined to a
nursing home awoke with a severe
head ache and stiff neck. Because
he had a high fever and signs of
meningitis,the nursing home staff
took him to a local emergency
department. The CSF specimens
cloudy. Find the organism

Haemophilus influenza

An 43 years Old woman Mrs. Latha
was admitted with the symptoms of
high fever,chills,head ache,pain in
chest,breathing difficulty and cough
with blood for 1-3days.What I’d the
causative organism?

Yersinia pestis

A 77 years old woman with
silence access and admitted in
the hospital. She is having
wound infection cellulitis and
food poisoning also. Find out
organism cause the disease

Clostridium perferigens

Patient with 40 years old have
complaints of skin
lesions ,numbness of the limbs and
he is insensitive to pain to touch
and nerve enlargement around
elbow and knee from above clinical
manifestations and find out the
name of the disease

Leprosy

Mrs. Geetha having a painful
spasm,muscle rigidity in neck ,
painful swallowing in past 3
weeks. Find out the causative
organism

Clostridium tetani

Match the names of scientists given in
Column A with the
 discovery made by
them given in Column B.

Match the names of scientists given in
Column A with the
 discovery made by
them given in Column B.
Column A Column B
Louis Pasteur Fermentation
Robert Koch Anthrax bacterium
Edward Jenner Small pox vaccine
Alexander Fleming Penicillin

3(2). Match the microorganisms given
in the Column A to the group
 to which
they belong in Column B.

Match the microorganisms given in
the Column A to the group
 to
which they belong in Column B.
Column A Column B
Lactobacillus Bacteria
Aspergillus Fungi
Spirogyra Algae
Paramecium Protozoa

Classify the following into friendly and
harmful microorganisms.
 Yeast,
malarial parasite, Lactobacillus, bread
mould, Rhizobium,
 Bacillus anthracis

Classify the following into friendly and
harmful microorganisms.
 Yeast,
malarial parasite, Lactobacillus, bread
mould, Rhizobium,
 Bacillus anthracis
Friendly Harmful
Yeast Malarial parasite
Lactobacillus Bread mould
Rhizobium Bacillus anthracis

Theme: Colony appearance
•Draughtsman/carum coin – Anthrax
•Medusa Head - Yersinia
•Stalactite growth- Pneumococci

Yersinia

Theme: Colony appearance
•Draughtsman/carum coin - Pneumococci
 
   
•Medusa Head - Anthrax
 
  
•Stalactite growth - Yersinia

Classify bacteria based on oxygen
requirement

54

Theme: Colony appearance
•Fried egg
•Bisected pearls
•School of fish
•H.ducreyi
•B. pertusis
•Mycoplasma

Theme: Selective media
•Buffered charcoal yeast extract agar-legionella
•Skirrow's medium - campylobacter
•TCBS agar - vibrio
•Mannitol salt agar medium -
staphylococcus

Theme: Selective media
•Buffered charcoal yeast
extract agar
•Skirrow's medium
•TCBS agar
•Mannitol salt agar
medium
•Staphylococcus
•Legionella
•Campylobacter
•Vibrio

3(8)Theme: Treponemal diseases
•Syphilis
•Yaws
•Pinta
•Bejel
•Treponema
pallidum
 endemicum
•T.pallidum
•T.pallidum carateum
•T.pallidum pertenue
BEJEL

Theme: Treponemal diseases
•Syphilis
   :  T.pallidum
•Yaws
    
:
 T.pallidum pertenue
•Pinta
    
:
 T.pallidum carateum
•Bejel:Treponema pallidum
 endemicum

A. Which is NOT a HACEK group of
organism
•Haemophilus aphrophilus
•Acenetobacter boumani
•Cardiobacterium hominis
•Eikenella corrodens

Which is NOT a HACEK group of
organism
•Haemophilus aphrophilus
•Acenetobacter boumani
•Cardiobacterium hominis
•Eikenella corrodens

1(2). What flagella arrangement is
exhibited in the image below?

Lophotrichous flagella

1(3). What 3 types of hemolysis are
shown in letters A, B, and C below?

A=Alpha B=Beta C=Gamma

1(4). The image below is a picture of
Clostridium tetani, the causative agent of
tetanus. What unique morphological
feature is seen in the image?

Terminal Endospores

1(5). What type of
genetic exchange is
being exhibited by the
E. coli in the picture
below?
What is the red
structure connecting
the two cells
together?

Conjugation; Sex Pili

1(6). This is a Staphyococcus aureus
isolate from a patient presenting with
cellulitis. The disc on the left contains
erythromycin and the disc on the right
contains clindamycin
What phenomenen is
shown here?

This is a D-zone showing that the
erythromycin has induced the Staph.
aureus to be resistant to clindamycin

1(7). Naegler's reaction is seen in
C.perfringens. Toxin responsiple is
Alpha
Beta
Gamma
Delta

Naegler's reaction is seen in
C.perfringens. Toxin responsiple is
Alpha
Beta
Gamma
Delta
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