Recombinant-DNA-Technology.pptx

RDNA

1. RECOMBINANT DNA Khwaja Moinuddin chishti Language University Department of bioetchnology Seminar Speaker – sparsh tiwari ( B.tech biotechnology)

2. PROPERTIES O F A GOOD R ec o mb i nant VACCINE ▶ A b i li t y t o e l i c i t t h e a p p ro p r i a t e i m m u n e r e s p o n s e for a p a r t i cu l a r p a t h og e n . ▶ L on g t e rm pr o t e c t i on ▶ Safety ▶ Stability ▶ inexpensiveness

3. RECOM B INAN T VACCINE ▶ A recombinant vaccine is a vaccine is produced by r - DN A t e c h n o l og y . ▶ This involves inserting the DNA encoding an antigen t h a t s t i mu l a t e s a n i m m u n e r e s p o n s e i n t o mammalian cells or bacterial cells ;expressing the a n ti gen i n t h e s e c e ll s an d p u r i f y i n g i t fr o m t h e m. ▶ First recombinant vaccine –Hepatitis B vaccine

4. Types of Recomb i nant vaccines ▶ Subunit recombinant vaccines – includes proteins, peptides and dna ▶ Attenuated recombinant vaccines –these genetically modified pathogenic organism(bact. , virus) that are made of non pathogenic a n d use a s v a cc i n e s. ▶ Vector recombinant vaccines- these are genetically modified viral v e c t ors t h a t v a n be us e d a s v a cc i n e s a ga i n s t cer t a i n pa t h og e n s .

5. Process i ng of rec o mb inant DNA Technology ▶ I s o l a ti on of t h e ge n e ti c ma t e r i a l ▶ Cu t ti n g of D NA a t Sp e c i f i c l oca ti on ▶ A mp l i f i c a t i on of Ge n e of i n t e r e s t us i n g PCR ▶ Inserting of Recombinant DNA into the Host cell/organism ▶ O b t a i n i n g t h e f o r e i gn g e n e p r od u ct

6. H O W D N A V A C CI N E IS M A DE E x pression plasmid Plasmid with foreign gene Viral gene Recombinant DNA Technology

7. B acterial cell Transform into bacterial cell Plasmid DNA

8. Plasmid DNA get Amplified

9. Plasmid DNA Purified Ready to use

10. Advantages of the recombi nant vaccine ▶ Produc e d more qu i ck l y ▶ I n l a rg e r q u a n titi e s ▶ Free from i n f e c t i o u s v i r a l p a r t i c l e s. ▶ They can safely be given to immunosuppressed animal. ▶ T h e y a re l e s s l i k e l y t o i n duc e s i de e ff e c t s