Recombinant growth hormone

this power point slides are made using the data obtained from various websites. some has been mentioned in the reference

1. Recombinant growth hormone : concept and application PRESENTED BY S.SRIVIDHYA III BIOCHEMISTRY

2. Growth hormone Somatotropin From somatotrophs Protein hormone 190 amino acids Growth, metabolism and drug

3. concept Every protein has mRNA so as DNA cloning

4. steps h GH isolated from pituitary gland. Gene is cleaved using restriction endonuclease. Add gene to a plasmid vector (carrier molecule) by cleaving using ligase. Construct with desired gene is obtained called rDNA . Plasmid under self replication. Introduced into E.coli cell. Gene expresses.

5. procedure Synthesis of first 24 nucleotides chemically with known sequence. (8X3=24) Isolate growth hormone from human pituitary gland. cDNA is formed using reverse transcription-gene for hGH production.

6. First 24 sequence is removed using restriction endonuclease. Synthetic gene and cDNA are joined using T4 ligase. Suitable vector is chosen. DNA joined to plasmid by endonuclease and ligase. Kept at Lac operon- operator region. Selection of host cell : E.coli. Recombinant   E. coli isolated from culture and mass production by fermentation technology to obtain hGH.

7. application GH deficiency treatment Athletics supplement Growth deficiency Tumer’s syndrome and chronic renal insufficiency treatment Renal carcinoma treatment Bovine somatotropin - increases milk production in lactating cows

8. advantages Time conservation Cost effective Cheap More copies due to recombinant technology

9. Limitation Transformation of diseases. Bone and heart problem Fatigue Muscle weakness Risk of type 1 diabetes

10. reference Shomu’s biology: https:// www.youtube.com/watch?v=YcwdptGhpVU Lifestyle medicine: https:// www.youtube.com/watch?v=wdAKp1P_Z2w http://www.onlinebiologynotes.com/production-human-growth-hormone-hgh-recombinant-dna-technology/