ROLE OF Growth factors IN PERIODONTAL REGENERATION.pptx

Role of growth factors in periodontics

1. ROLE OF Growth factors IN PERIODONTAL REGENERATION

2. HISTORY Rita Levi- Montalcini ( 1909- 2012) Stanly Cohen (1922-Alive)

3. Growth factors A substance made by the body that functions to regulate cell division and cell survival. “Dictionary of cancer terms” The chemical messengers that induce cell growth by tissue type. “Mosby’s dental dictionary A family of polypeptide biological mediators which modulate cell responses such as cell chemotaxis, adhesion, survival, proliferation, and differentiation. Periodontology 2000, 2009

4. Regeneration -refers to complete restoration of functional supporting tissues including the alveolar bone, the cementum , and the periodontal ligament.

5. Growth factors v/s morphogens Growth factors = osteogenic Morphogens = Osteoinductive The advantage of utilizing morphogens like BMP versus growth factors in the (re)generation of bone is that the pre-existence of osteprogenitor cells in the area is unnecessary.

6. CLASSIFICATION Platelet concentrates 1 st generation Autologous fibin glue Platelet rich plasma (PRP) Pure (P-PRP) Leukocyte rich (L-PRP)

7. 2 nd generation Platelet rich fibrin (PRF) Pure (P-PRF) Leukocyte (L-PRF) Titanium PRF (T-PRF) Advanced (A-PRF) Injectable PRF ( i -PRF) Concentrated growth factors (CGF)

8. Marx first used PRP in the mandibular reconstruction defects It is a FIRST GENERATION platelet concentrate .. It contains PLATELET RICH PLASMA

9. 3500rpm for 15 mins Platelet- poor plasma Platelet- rich plasma CENTRIFUGATION P ROTOCOL 1) 2) 3)

10. LIMITATIONS

11. Devoid of Bovine thrombin The combination of fibrins and cytokines within PRF becomes a powerful bio scaffold with an integrated reservoir of growth factors for tissue regeneration GENERATION

12. Platelet Poor Plasma ( PPP) Platelet Rich Fibri n (PRF) RBC

13. First developed in France by CHOUKROUN Large quantity of leukocytes embedded in fibrin matrix.

14. Why L-PRF ???

15. ADVANCED PLATELET RICH FIBRIN

16. CENTRIFUGATION PROTOCOL

17. A –PRF PRF

18. The same PRF concept as no anticoagulant neither an additive It coagulates immediately after the injection. INJECTABLE PLATELET RICH FIBRIN

19. Blood collection Spin Separation collection of I - PRF I PRF + BONE GRAFT

20. STICKY BONE

21. Sohn et al 2010 fabricated growth factors enriched bone graft matrix and called it as Sticky bone.

22. Autologus Fibrin Bone Graft YELLOW STICKY BONE 2400-2700 rpm 2mins

23. CGF RED STICKY BONE YELLOW STICKY BONE 2400-2700 rpm 12mins

24. THANK YOU