Role of Tissue culture in Poinsettia plant

informartion about tissue cultue in poinsettia

1. ODISHA UNIVERSITY OF AGRICULTURE AND TECHNOLOGY College of Agriculture Bhubaneswar SUBMITTED BY: RAYAPU.SAITHEJA M.Sc. (Ag.), Floriculture and Landscaping BIOTECHNOLOGY IN POINSETTIA SUBMITTED TO:- Dr. Siddarth Kumar Palai Professor &Head Department of Floriculture and Landscaping, College of Agriculture, OUAT Bhubaneswar

2. Poinsettia Euphorbia spp

3. Introduction Scientific name : Euphorbia spp Family : Euphorbiaceae Origin : Mexico & Central America Leaves : Uppermost leaves of the plants resembles flower. Color - red, white, pink Flower : Small in size and resembles the stamen of the flower. Remains intact for 3-4 months. Propagation : Seeds or cuttings of plants. Fast-growing shrub and one of the most common growing shrubs worldwide. Most popular pot plant among gardeners and plant enthusiasts. The genus Euphorbia consists of around 2000 species of flowering plants.

4. Limitations of growing Poinsettia using Traditional methods The slow growth rate of plantlets. Few micro shoots per explant. Slow root growth rate Seeds lose viability during storage. Genetic variability . Poinsettia industry needs approaches for Rapid multiplication of elite clones. The production of pathogen-free plants. More rapid introduction of novel cultivars ( Cvs .) with desirable traits. This can only be achieved by using the advanced micro propagation technique.

5. Tissue culture is an advanced technique for growing plants. It requires only a few tissues of plants to regenerate a whole plant in complete aseptic conditions. The technique allows growers to multiply plants rapidly in small spaces, produce disease-free plants, and preserve them for a long time. MICROPROGATION

6. Take a healthy mother plant of E. pulcherrima ‘ Prestige Red ’ and excise axillary or apical buds from the plant. Surface sterilize the explant by keeping it in a 1% Tween 20 solution for 5 minutes followed by transferring it to 70% ethyl alcohol for 1 min. Then, transfer the explant to 20% bleach for 15 minutes. Rinse the explant in sterile water three times to remove any residue of the chemicals. Prepare a culture media using MS media (4.4 g/L), Myo -inosito l (0.1 g∙L-1), Sucrose (30 g∙L-1) and Agar (7 g∙L-1) (pH 5.7–5.8). Then, add 4 μM BA 6-benzylaminopurine . Pour the liquid media into 100 ml baby food jars and autoclave them at a temperature of 121 ºC at 15 psi pressure for 15 min . STEP WISE PROCEDURE

7. After the media is sterilized, take out the culture bottle safely from the autoclave, let it cool down, and place the surface sterilized explant in the media-containing jars. Incubate the culture bottles in a growth chamber with 16-h photoperiod (125 μmol∙m-2∙s-1 illuminations) at 25 ºC day and 22 ºC night temperatures . Keep sub culturing the explant in the same media recipe for four months. After four months, plants will be ready to transfer to the rooting media for root generation. Prepare rooting media by taking ½ strength MS media, Myo -inositol (0.1 g∙L-1), sucrose (30 g∙L-1), and agar (7 g∙L-1) (pH 5.7–5.8) with 28.5 Μ m. Transfer the regenerated shoots of the plant to the rooting media.

8. In 3-4 weeks , you will have Poinsettia plants with well-developed root systems and ready to be acclimated to greenhouse conditions.

9. Thankyou