Rose bengal plate test

brucellosis diagnosis
agglutinin reaction
field test
easy test

1. ROSE BENGAL PLATE TEST ABHIJITH SP CVAS POOKODE

2. Introduction Brucellosis is a worldwide zoonosis caused by the bacterial genus Brucella. These organisms localize in the reproductive organs of host animals, causing abortions and sterility. They are shed in large numbers in the animal's urine, milk, and placental fluid. Exposure to infected animals and animal products causes the disease in humans.

3. Introduction Brucellosis was first diagnosed by Wright and Smith in 1897. Rose Bengal test detects antibodies. The synonyms of Brucellosis are: 1.Undulant fever 2.Malta fever. 3.Gibraltar fever. 4.Mediterranean fever

4. Rose Bengal Plate Test It is a rapid plate agglutination test by using a slide. Used in field for diagnosis of Human brucellosis and Bovine Brucellosis. It is actually a screening test and positive reaction samples should be confirmed by STAT or CFT. Time taken for reaction is 2 minutes. Cheap and Faster. Synonyms: Rose Bengal Agglutinin Test, Rose Bengal Dye Test

5. Materials Required 1.Rose Bengal Dye stained antigen 2.Micropipette 3.Microscopic slides 4.Applicator sticks. 5.Gloves

6. Procedure A clean and sterilized slide is placed on a white background. Rose Bengal Dye stained Antigen vial should be gently swirled. With a micropipette two drops of antigen are taken on both ends of glass slide. An equal volume of test sera is added to the antigen. With a non-metallic stick, gently mix the antigen and sera. Rotate slide is slanted orbital motion. Rotate the slide with a mechanical rotator at 80-100 rpm for 2 minutes (if possible). Appearance of agglutinin clumps indicate positive reaction. Positive samples should be confirmed by STAT or CFT.

7. Result

8. Result and Interpretation No agglutination= absence of specific antibodies Agglutination (even slight) = presence of specific antibodies If agglutination appear after 15 seconds = (1:640) If agglutination appear after 30 seconds = (1:320) If agglutination appear after 1 min. = (1:160) If agglutination appear after 1.30 min. = (1:80)

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