Seminario Biología Molecular JyF 2024-02.

Se evalua el artículo: TSP50 facilitates breast cancer stem cell-like properties maintenance
and epithelial-mesenchymal transition via PI3K p110α mediated activation of AKT signaling
pathway

1. Maria Fernanda Pineda Garavito
Justo Alejandro Porrras
Molecular BiologyMolecular Biology
Third Semester
UPB
Third Semester
UPB

2. Introduction
Breast Cancer Threonine-Serine Protein
Kinase 50 (TSP50)
BRCA1 and BRCA2 genes

3. Introduction

4. To correlate TSP50 levels with
clinical and prognostic parameters
in breast cancer patients to
determine its relevance as a
biomarker.
To analyze TSP50 expression in
different breast cancer cell lines and
tumor tissue samples to establish its
relationship with EMT.
To evaluate the functional impact of
TSP50 through gain and loss of
function experiments, observing
changes in cell invasion and
proliferation capacity.
General objectives

5. Flow cytometry

CD44 - PE CD24 - APC

6. Immunoprecipitation IP

A/G protein
Anti-TSP50, anti-p110α
and anti-flag.

7. qRT-PCR (Real Time PCR)

8. WESTERN BLOT
O INMUNOBLOT

9. FIGURE 1

10. FIGURE 2

11. FIGURE 3

12. FIGURE 4

13. Author Opinion Suppor or Refuse
Song ZB
The level of TSP50 holds significant clinical implica-
tions for individuals with breast cancer
Ferlay J
Breast cancer, which is the second leading cause of
can- cer-related mortality, maintains to be a
significant burden for women globally
Yu J
Studies have shown that the p85 subunit attaches to
the p110 subunit and suppresses its catalytic activity
at rest or in the absence of stimulation
Discussion

14. TSP50 not only promotes epithelial-
mesenchymal transition (EMT), but also
potentiates cancer stem cell characteristics,
important for addressing tumor
heterogeneity and therapy resistance.
The identification of TSP50 as a key
regulator in epithelial-mesenchymal
transition (EMT) highlights its importance in
the molecular biology of breast cancer,
suggesting that its modulation could
influence tumor progression.
1.
Conclusions
2.
3.
TSP50 could be a prognostic biomarker, as its
high expression is associated with worse
prognosis and could guide therapeutic decisions
in breast cancer management.

15. Maria Fernanda Pineda - Mindmap

16. Justo Alejandro Porras- Mapa conceptual