Single cell protein

Single Cell Protein

1. SINGLE CELL PROTEIN PRESENTED BY:- Sonika MSc

2. CONTENTS INTRODUCTION SINGLE CELL PROTEIN MICROORGANISMS BASIC STEPS OF SCP PRODUCTION ALGAE AS SCP BACTERIA AS SCP FUNGAL SCP YEAST SCP

3. INTRODUCTION Single cell proteins is the dried biomass of a single species of microbe that can be used as a protein source in the diet, is known as single cell protein . Biomass of certain algae, some fungi and some bacteria are used as single cell proteins.

4. MICROORGANISMS USED IN SCP PRODUCTION 1. ALGAE 2.BACTERIA 3. YEAST 4. FUNGI

5. MICROORGANISMS USED IN SCP PRODUCTION MUST BE 1. NON PATHOGENIC 2. GOOD NUTRITIONAL VALUE 3. EASILY AND CHEAPLY PRODUCED ON SCALE 4. TOXIN FREE 5. FAST GROWING 6. EASY TO SEPARATE FROM MEDIUM

6. MICROORGANISMS

7. ALGAE AS SCP Microalgae such as Spirulina , Chlorella, Scenedesmus , Dunaliella etc are consumed as Single Cell Protein . Algal SCP has about 60% crude protein. They are suitable for animal feeds or protein rich supplements.

8. SPIRULINA Spirulina is a spirally coiled, multicellular , filamentous blue green algae. Found in freshwater. Brackishwater , marine waters. Biomass is a rich source of protein, vitamins, minerals and beta carotene

9. MASS CULTURE OF SPIRULINA Cultured in large scales in artificial ponds, tanks, oxidation ponds. Three methods of culturing 1.Open circulatory system. 2.oxidation pond system. 3.photobioreactors(PBR).

10. 1 . OPEN CIRCULATORY SYSTEM Man-made open tank. May be circular or rectangular. Medium is filled in the open circulatory tank. Tank is inoculated with small volume of Spirulina . Culture is stirred continuously Spirulina grow rapidly.

11. 2.OXIDATION POND SYSTEM All solid wastes and suspended particles are removed from the sewage. Sewage water allowed to flow in to an oxidation pond. Spirulina culture is inoculated. Spirulina produce dense mat on the surface of sewage water. Biomass is harvested.

12. 3. PHOTOBIOREACTORS(PBRs) These are transparent tubes or containers. PBRs provide more controlled environment for better growth. PBRs offers the following advantages:- Easy maintenance of the algal culture. Protection from contaminations. Avoidance of water loss. Low cost of production.

13. USES As health food. As therapeutic agent. As a supplement animal feed. As a raw material for cosmetics . USES

14. BACTERIA Dried biomass of some bacteria is rich in protein. They can be grown in cheap renewable raw material and wastes. For ex:- Candida utilis , Candida lipolytica , Methylophilus methylotrophus , Cellulomonas etc are consumed as protein sources.

15. MASS PRODUCTION OF BACTERIAL SCP 1.FORMULATION OF MEDIUM :- Bacterial cells require a suitable carbon source, P,K, Na,H 2 and trace element for their growth. Other nutrients are added to it. And dilute properly to formulate culture medium.

16. CONTI…. 2 . FERMENTATION :- sterilized medium pumped in to air lift fermenter . Inoculation of bacterium. Mixing of sterile air. Broth is harvested.

17. RECOVERY OF BACTERIAL BIOMASS + Centrifuge HARVESTED BROTH WATER REMOVAL OF WATER

18. FUNGI AS SCP A few yeast or moulds are being used as SCP. Fungi grow rapidly on wide variety of cheap raw materials. Less amount of nucleic acid. Digestibility and acceptability of fungal protein is high. FUNGAL AS SCP

19. YEASTS Many yeast are consumed as SCP . Ex . Saccharomyces fragilis , Candida utilis , Candida lipolytica , Rodotorula glutinis , Torulopsis .

20. MASS CULTURE Can be grown by fed batch fermentation. Addition of inorganic materials such as N,P,K,Fe,Mg,AND S. Diluted with water and sterilization of medium. Medium is filled in fermenter . Inoculation of yeast. Sterile air is pumped. Broth is harvested.

21. Sterilized medium Bacterial suspension Centrifugation Drying Heat shock Toxicity test SCP fermenter water water Production of bacterial biomass for single cell protein

22. Reference Google Research paper

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