Skin tissue engineering

engineered tissue, skin grafting , apligraft

1. PROJECT TITLE SKIN TISSUE ENGINEERING NAME - NITINJAY SINGH ID - 17BTBIOCE011

2. CONTENT SKIN ANATOMY FUNCTION OF SKIN PROCESS OF WOUND HEALING SCAR FORMATION V/S REGENERATION SOLUTION WHEN WOUND NOT HEAL SKIN GRAFTING SKIN SUBSTITUTE APLIGRAFT

3. SKIN ANATOMY

4. FUNCTION OF SKIN HEAT REGULATION SENSATION SYNTHESIS AND STORAGE OF VITAMIN D EXTRACTION OF UNWANTED SUBSTANCE THROUGH SWEAT BLOOD RESERVOIR PROTECTION FROM FLUID LOSS AND ENVIRONMENT

5. PROCESS OF WOUND HEALING

6. SCAR FORMATION VS REGENERATION REGENERATION Tissues are able to replace damaged tissues and return to normal state Proliferation of cells(angiogenesis) Parenchymal organ eg : liver cell SCAR FORMATION Incapable of complete restoration Connective (fibrous) tissue Deposition of collagen eg : brain & heart cells

7. SOLUTIONS WHEN WOUND NOT HEAL INDUCED PRIMARY HEALING Stitches DELAYED PRIMARY HEALING Skin graft or engineered tissue

8. SKIN GRAFTING It is a surgical procedure in which skin or skin substitute is placed over a burn or non healing wound. Purpose Permanent replace damage skin Temporary wound repairing

9. CLASSIFICATION OF SKIN GRAFT SPLIT SKIN GRAFT Epidermis and small part of dermis layer Heals rapidly , good survival Skin harvest from back ,abdomen , thigh FULL THICKNESS GRAFT Both epidermis and dermis Resembles normal skin Abdomen, groin, forearm

10. RISK FACTORS FOR SPLIT SKIN GRAFT Loss of hair growth infection Poor sensation RISK FACTORS FOR FULL THICKNESS GRAFT Long healing time High risk of graft failure Donor sites are limited

11. SKIN SUBSTITUTE  Provide rapid wound coverage solution   Reduce or removed inhibitory factors of wound healing Reduced inflammatory response and  scarring

12. IDEAL SKIN SUBSTITUTE TISSUE COMPATIBILITY PERMEABLITY FLEXIBLE WIDELY AVAILABLE RESIST INFECTION POROSITY TO GROWTH FIBROVASCULAR TISSUE

13. CLASSIFICATION 1. ON THE BASIS OF LAYERS

14. ON THE BASIS OF DURABILITY TEMPORARY SKIN SUBSTITUTE PORCINE AMNION BIOBRANE PERMANENT SKIN SUBSTITUTE INTEGRA APLIGRAFT ALLODERM

15. APLIGRAFT PERMANENT BI –LAYER SKIN SUBSTITUTE Epidermal layer : keratinocytes Dermal layer : fibroblasts in bovine type 1 collagen Cells from human neonatal foreskin tissue

16. USES Chronic wounds Diabetic ulcers Epidermolysis Bullosa ADVANTAGES Readily available DISADVANTAGES Risk of disease transmission Expensive

17. The End Thank you